Banca de QUALIFICAÇÃO: PATRICIA INGRID MACEDO DE CASTRO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE: PATRICIA INGRID MACEDO DE CASTRO
DATA: 13/11/2015
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich - Depto. de Bioquímica - CB/UFRN
TÍTULO:

Proinflammatory cytokines in the pathogenesis of preeclampsia

PALAVRAS-CHAVES:

Preeclampsia, cytokines, pregnancy

PÁGINAS: 100
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Biologia Molecular
RESUMO:

Preeclampsia is disease affecting between 3 and 8% of a
pregnancy. The risk factors for this disease are not completely
understood. Dysregulation of the immune response could be a
role in the preeclampsia pathogenesis. This study aimed to
determine whether inflammatory cytokine receptors (IL1R, IL6R
e TNFαR) were associated with preeclampsia.Inflammatory
cytokine receptors IL1R, IL6R e TNFαR were assessed in
mononuclear cells from non-pregnant women (NP; n = 5),
healthy pregnant women (HP; n = 11) and pregnant women with
preeclampsia (PE; n = 24) using flow cytometry. Intermediate
monocytes (CD14highCD16+) were increased in preeclamptic
patients (7.14±6.38; 4.31%; p=0.0047) in comparison with
nonpregnant (1.36%±0.45; 1.22%). Regarding the non-classical
monocytes (CD14lowCD16+) or classical monocytes, no
differences were found in this population among the groups. In
comparison with nonpregnant women, Preeclampsia had
decreased TNFαR expression in intermediated monocyte (PE:
78.24%±21.5; 85.8% vs NP: 96.42%± 3.70; 98.2%; p=0.0471) and in nonclassical subpopulation (PE: 72.59%±30.97; 84.1% vs NP: 97.48%±1.57; 96.7%; p=0.0104). While, IL6R expression
were increased in intermediated (PE: 13.20%±28.13; 2.3% vs
NP: 0.07%±0.10; 0.0%; p=0.0022) and in nonclassical
monocytes (PE: 28.5%±30.81; 16.3 vs NP: 2.42±2.17; 3.16;
p=0.042; and PE: 28.5%±30.81; 16.3 vs HP: 0.91%±0.96;
0.55%; p=0.0085). In lymphocytes, was observed the same
pattern for TNFR and IL6R in preeclamptic patients. TNFαR
decreases the expression in CD3+CD4+ (PE: 21.12%±22.3;
13.9% vs NP: 52.32±5.93; 54.5%; p = 0.0168) and CD3+CD8+
(PE: 18.69±22.0; 11.8% vs NP: 48.72%±9.18; 45.8%; p =
0.0197). However, a significant increase of IL-6R expression
was observed only in CD3+CD8+ subpopulation (PE:
22.55%±22.26; 18.8% vs NP: 0.29%± 0.17; 0.28%; p = 0.0018).
No differences for IL1R were found in monocytes and
lymphocytes subpopulations.

MEMBROS DA BANCA:
Externo ao Programa - 1714243 - DANIELLA REGINA ARANTES MARTINS SALHA
Externo ao Programa - 1212336 - ELIANA LUCIA TOMAZ DO NASCIMENTO
Externo ao Programa - 350753 - VALERIA SORAYA DE FARIAS SALES