Banca de QUALIFICAÇÃO: NATASHA LORENNA FERREIRA DA SILVA LEAL

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : NATASHA LORENNA FERREIRA DA SILVA LEAL
DATE: 15/05/2025
TIME: 08:00
LOCAL: VIDEOCONFERÊNCIA
TITLE:

Phenolic

-rich extrac

t from Bryophyllu

m pinnatum leaf cultivated unde
r

drough
t stress conditions modulate
s th
e antioxidan
t an
d inflammation

response

 

KEY WORDS:

Kalanchoe pinnata, saião, active ingredient, phenolic compounds, flavonoids, phytotherapy, sustainability.

PAGES: 81
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Bryophyllum pinnatum (Lam) Pers (Crassulaceae) is used in traditional medicine to treat

inflammatory diseases. There is preclinical and clinical evidence proving its anti-inflammatory

effect. This species is a good source of phenolic compounds, rich in flavonoids, with quercetin

3-O-L-arabinopyranosyl-(1→2)-O-L-rhamnopyranoside (Bp1) being its major compound.

However, in order to obtain a standardized raw material as an active pharmaceutical ingredient,

it is necessary to standardize the production chain from cultivation to the final product, ensuring

reproducibility. The objective of this study was to understand whether water and salt stresses

are capable of increasing the phenolic content in the leaves of B. pinnatum to obtain an extract

rich in phenolics, and whether the extracts obtained after these two stress methods were capable

of modulating the anti-inflammatory and antioxidant responses. B. pinnatum was subjected to

different periods of water stress during the cultivation conditions with the following

experimental groups (n = 10): T5-T30 groups (5 to 30 days without irrigation); T5c-T30c

control groups subjected to daily irrigation; cyclic group of 10 days without irrigation and 10

more daily irrigations. And under saline stress, the individuals received daily irrigation with

water added with NaCl for 30 days, at concentrations of 50 mM, 100 mM and 200 mM, being

the groups G1, G2 and G3 respectively. After the treatment, dry extracts of the leaves of each

sample were prepared. Subsequently, the total flavonoids (TFC) and total phenolics (TPC)

contents were analyzed by means of colorimetric tests. Ultrafast liquid chromatography coupled

to a diode array detector (UPLC-DAD) was used to quantify the major compound (Bp1) in the

extracts. The cytotoxicity and proliferative activity in RAW 264.7 cells and the in vitro

antioxidant activity were evaluated by means of Total Antioxidant Capacity (TAC), Reducing

Power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) tests. To evaluate the pharmacological

activity, in vivo models of croton oil-induced ear edema and carrageenan-induced paw edema

were performed in Swiss mice. The quantification of nitrite and MPO enzyme activity were

evaluated in ear and paw samples collected from the two preclinical models performed. Extracts

under different stress conditions showed no signs of cytotoxicity (RAW 264.7 cells) and were

able to donate electrons in in vitro antioxidant assays (TAC, reducing power and DPPH).

Extracts with high TPC (T20t and T30t) showed anti-inflammatory activity in the ear edema

model induced by croton oil at a dose of 200 mg/kg orally, as well as significantly reduced

nitrite levels and MPO enzyme activity in mouse ear samples when compared to the control

group. In the paw edema model induced by carrageenan, treatment with the extracts did not

inhibit edema, nor was it able to reduce nitrite levels and MPO enzyme activity in the paws of

the mice tested. Regarding the Bp1 content, no major differences were observed between the

groups. The results demonstrate that water stress is a promising and sustainable strategy for the

cultivation of B. pinnatum aiming to increase the phenolic content and positively modulate its

pharmacological effects, while salt stress had no influence on the increase in phenolic content

in the cultivation of this species. Therefore, phenolic-rich extracts from B. pinnatum can be

obtained with low water consumption, being a good source of raw material to be used as an

active ingredient in herbal medicines or supplements.

COMMITTEE MEMBERS:
Presidente - 2323511 - ADRIANA AUGUSTO DE REZENDE
Externa ao Programa - 1143827 - JULIANA FELIX DA SILVA - nullExterna à Instituição - LARISSA MARINA PEREIRA SILVA - SESAP