Banca de QUALIFICAÇÃO: LUANDA BARBARA FERREIRA CANARIO DE SOUZA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE: LUANDA BARBARA FERREIRA CANARIO DE SOUZA
DATA: 30/09/2014
HORA: 09:00
LOCAL: SALA DE AULA II 2º ANDAR DO PPGCF
TÍTULO:

EFFECT OF THE CRUDE EXTRACT OF Eugenia uniflora ON ADHESION TO HUMAN BUCCAL EPITHELIAL CELLS AND BIOFILM FORMATION OF Candida spp. FROM THE ORAL CAVITY OF KIDNEY TRANSPLANT RECIPENTS.


PALAVRAS-CHAVES:

Candida spp., oral candidiasis, kidney transplant recipients, virulence factors, Eugenia uniflora


PÁGINAS: 42
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Candidiasis is a major oral manifestation in kidney transplant patients. Candida spp. possess essential virulence factors which contribute for the infectious process, including the ability to adhere to epithelial cells and biofilm formation. The crude extract obtained from the leaves of Eugenia uniflora has demonstrated antifungal activity against Candida spp. This study evaluated the influence of the crude extract of E. uniflora in adhesion to human buccal epithelial cells (HBEC) and biofilm formation of 43 strains of Candida spp. isolated from the oral cavity of kidney transplant patients. For the virulence tests evaluated in vitro, yeasts were grown in the presence and absence of 1000 g/mL of the extract. A ratio of 10: 1 (Candida spp. cells X HBECs) was incubated for 1 hour at 37 ° C, 200rpm, fixed with 10% formalin and the number of Candida cells adhered to 150 CEBH determined by optical microscope. Biofilms were formed on polystyrene microplates in the presence of the crude extract The quantification of biofilms was performed with crystal violet staining, visualized at 570 nm. We could detect that the crude extract of E. uniflora was able to reduce adhesion to HBEC for both Candida albicans and non-Candida albicans Candida species. On the other other hand, only 16 Candida spp. strains (36%) showed reduced biofilm formation. However, two highly biofilm producer strains of C. tropicalis had a notorious reduction in biofilm formation. This study reinforces the idea that besides growth inhibition, E. uniflora may interfere with the expression of some virulence factors of Candida ssp., and may be possibly applied in the future as a novel antifungal agent.


MEMBROS DA BANCA:
Presidente - 3313589 - JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS
Interno - 1871916 - RAQUEL BRANDT GIORDANI
Externo ao Programa - 1149398 - EVELINE PIPOLO MILAN
Notícia cadastrada em: 29/09/2014 15:06
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