Banca de DEFESA: YOHANNA LAYSSA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : YOHANNA LAYSSA DOS SANTOS
DATE: 30/04/2024
TIME: 14:00
LOCAL: DNUT e google meet
TITLE:

TOXICITY OF TRYPSIN INHIBITOR ISOLATED FROM TAMARIND
SEEDS (TTI) IN ZEBRAFISH

KEY WORDS:

Danio rerio; Embryotoxicity; cardiotoxicity; neurotoxicity; genotoxicity;
Tamarindus indica L.

PAGES: 60
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

The trypsin inhibitor isolated from tamarind seeds (TTI) has been
investigated for potential applications in the treatment of non-

communicable chronic diseases (NCDs). However, in vivo
toxicological safety throughout ontogeny needs to be investigated to
ensure the proposed applications. Therefore, this study aimed to
evaluate embryotoxicity and acute toxicity in adult zebrafish (Danio
rerio) exposed to TTI at different concentrations. Initially, TTI was
obtained from tamarind seeds through protein fractionation using
ammonium sulfate, followed by affinity chromatography on Trypsin-
Sepharose CNBr 4B and characterized for molecular mass and anti-
trypsin activity. Fish, both in the embryonic and adult stages, were
exposed for 96 hours post-fertilization (hpf) to three concentrations of
TTI (12,5, 25 and 50 mg/L). Zebrafish embryos (n=20 animals per
group) were investigated for survival, hatching, malformations, and
possible changes in developmental endpoints, as well as cardiotoxicity
and neurotoxicity tests. To assess acute toxicity in adult animals (n=20
animals per group), neurotoxicity investigation was conducted through
avoidance tests, light-dark and novel tank tests, as well as
genotoxicity and histopathology. Regarding the tests performed,
zebrafish embryo groups exposed for 96 hpf to TTI (12,5, 25 and 50
mg/L) showed no adverse effects (p>0,05) compared to animals
exposed to 3,4-dichloroaniline (p<0,0001). Additionally, these same
animals maintained heart rate within the normal range (152, 157, 171
bpm respectively), without showing adverse effects. TTI did not cause
mortality or significant behavioral changes in adult zebrafish,
suggesting the absence of neurotoxicity. Additionally, genetic tests
revealed no signs of genotoxicity, and the evaluated tissues (liver,
intestines, and kidneys) showed no histopathological changes, with
preserved structures. Therefore, these results are robust and indicate
the absence of toxic properties of TTI in zebrafish at different
developmental stages, thus reinforcing the established understanding
of its safety and efficacy, which is of great relevance to the scientific
community. Thus, the results present new approaches for research
and applications related to TTI.

COMMITTEE MEMBERS:
Externo à Instituição - ALEXANDRE COELHO SERQUIZ - UFPB
Externa ao Programa - 1644341 - ANA CAROLINA LUCHIARI - nullPresidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externa ao Programa - 1674112 - RENATA ALEXANDRA MOREIRA DAS NEVES - null