Banca de QUALIFICAÇÃO: BEATRIZ SILVA LOPES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : BEATRIZ SILVA LOPES
DATE: 28/02/2024
TIME: 09:00
LOCAL: Departamento de Nutrição da UFRN e Via Google Meet
TITLE:

TOXICOLOGICAL SAFETY OF HYDROALCOHOLIC EXTRACT OF JAMBOLÃO (Syzygium Cumini (L.) Skeels) IN ZEBRAFISH (Danio rerio)

KEY WORDS:

Syzygium cumini; anthocyanins; toxicological screening; Danio rerio.

PAGES: 60
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

The jambolan plum (Syzygium cumini (L.) Skeels) is considered an important source of phenolic compounds, especially anthocyanins, a class of phenolic compounds that contribute to the biological properties of the fruit. However, prior to studying the bioactive effects of jambolan plum, it is necessary to conduct investigations to ensure safe dosages for use, especially when using extracts that concentrate phytochemicals. In this regard, the study aimed to evaluate the toxicological safety of the hydroalcoholic extract of jambolan plum through the zebrafish (Dario rerio) model, administered at different concentrations and life stages of this animal. The jambolan plum extract (JPE) was obtained by mixing lyophilized powder from the fruit (seedless) of jambolan plum in a solution of ethanol (70%) and water (30%), followed by an exhaustive extraction process with agitation at room temperature, centrifugation, and filtration. The JPE was subjected to high-performance liquid chromatography (HPLC) and characterized for total phenolic compounds (TPC), total monomeric anthocyanins (TMA), and antioxidant activity. Fifteen phenolic compounds were identified in the extracts by HPLC, with a highlight on the anthocyanins malvidin 3,5-diglucoside, delphinidin 3- galactoside, and cyanidin 3,5-diglucoside. The amounts found were 21.80 ± 0.01 mg GAE/g fruit for TPC and 11.39 ± 0.91 mg Cy3-G/g fruit for TMA. Antioxidant activity was evaluated by the ABTS+ (323.88 ± 11.05 mmol Trolox Eq./g fruit) and DPPH (844.70 ± 58.81 mmol Trolox Eq./g fruit) methods. After the extract characterization, zebrafish in the embryonic and adult stages were exposed to various concentrations of JPE (10 – 400 µg/mL) and subjected to acute toxicological screening, cardio, and neurotoxicity evaluation. Additionally, ongoing morphological and histological analyses are in progress. Concentrations of JPE (10, 25, and 50 µg/mL) proved to be safe for treating larvae throughout the experiment, while values above 50 µg/mL caused malformations and death. The estimated LC50 for embryos was 114.63 ± 6.14 µg/mL, and the concentration of 400 µg/mL was considered lethal for 100% of the larvae. Based on these values, the LC50 was determined (118.4 µg/mL). No cardio or neurotoxic alterations resulting from exposure to JPE were observed in the evaluated larvae. The LC50 for adult animals was estimated at 70.17 ± 6.82 µg/mL, and currently, tissue and behavioral analyses are in the execution phase. At the end of the study, it is expected to obtain data regarding therapeutic doses of JPE and the optimal administration time that do not present toxic effects on the analyzed parameters, aiming to advance research on the bioactive potential of this extract in different health conditions.

COMMITTEE MEMBERS:
Externa ao Programa - 1644341 - ANA CAROLINA LUCHIARI - nullPresidente - 1971809 - JULIANA KELLY DA SILVA MAIA
Externa à Instituição - LILIAN CRISTINA PEREIRA - UNESP
Interna - 2275877 - THAIS SOUZA PASSOS