Banca de DEFESA: KARINA ZAIRA SILVA MARINHO COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KARINA ZAIRA SILVA MARINHO COSTA
DATE: 24/03/2023
TIME: 09:30
LOCAL: Sessão pública realizada por videoconferência
TITLE:

Differential microRNA expression and its association between plasma micronutrients and inflammatory biomarkers in overweight women with hyperglycemia

KEY WORDS:

microRNA, hyperglycemia, inflammation, micronutrients, overweight

PAGES: 67
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Hyperglycemia is important in the development and maintenance of chronic inflammation and oxidative stress in overweight individuals and is a risk factor for cardiovascular disease, metabolic syndrome, and complications resulting from type 2 diabetes mellitus. Nutrients with anti-inflammatory and antioxidant action can interact with the genome, modulating its expression, resulting in alterations in the phenotype and the risk markers for chronic diseases. This interaction allows the modulation of microRNA (miRNA) expression, which are important for the regulation of several physiological or pathological processes, such as hyperglycemia. Therefore, this study aimed to identify miRNA differentially expressed in overweight women with hyperglycemia and its association with inflammatory biomarkers and plasma micronutrients. This is a cross-sectional comparative study derived from a study with 72 women between 18 and 60 years old and BMI ≥ 25.0kg/m². In plasma samples, zinc, selenium, and manganese concentrations were evaluated, as well as fasting glucose, insulin, miRNA expression, inflammatory biomarkers, and leptin. Insulin resistance was assessed by calculating the HOMA-IR and HOMA-β indices. Most participants did not have hyperglycemia (68.1%). miR-29a-3p, miR-150-5p, and miR-548c-5p were upregulated in hyperglycemic women compared to normoglycemic. A positive correlation was observed between miR-150-5p expression and IL-10 (rho=0.348; p=0.030) and a negative correlation with leptin (rho=-0.331; p=0.040), as well as a negative correlation between miR-548c-5p and HOMA-IR (rho=-0.424; p=0.022) and HOMA-β (rho=-0.463; p=0.011) in normoglycemic women. miR-29a-3p and miR-548c-5p were positively associated with hyperglycemia (OR=2.34; p=0.024 and OR=109.71; p=0.016, respectively). No differences were identified for zinc, selenium, and manganese concentrations in plasma between women with and without hyperglycemia, as well as correlations between these micronutrients and upregulated miRNAs. Pathway enrichment analysis of target genes identified that these miRNAs are potentially related to glycemic metabolism, the pathophysiology of hyperglycemia, and diabetes, or complications of the disease. Given this, it is understood that these miRNAs have the potential to be used as biomarkers of hyperglycemia and its complications, in addition to being involved in the activation of inflammatory pathways in these patients. Furthermore, the results of this study suggest that the micronutrients zinc, selenium, and manganese do not seem to be related to the expression of these miRNAs.

COMMITTEE MEMBERS:
Presidente - 3218336 - BRUNA ZAVARIZE REIS
Externo à Instituição - ERNESTO VARGAS MENDEZ - UCR
Interna - 2306763 - KARINE CAVALCANTI MAURICIO DE SENA EVANGELISTA
Externo à Instituição - MARIA ADERUZA HORST - UFG