Banca de DEFESA: LEDA KARLA MONTEIRO DIAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LEDA KARLA MONTEIRO DIAS
DATE: 22/11/2022
TIME: 09:00
LOCAL: Sessão pública realizada por videoconferência
TITLE:

POLYPHENOLS EFFECT ON INTESTINAL HEATH OF PRECLINICAL STUDIES WITH DIET-INDUCED OBESITY: SYSTEMATIC REVIEW AND META-ANALYSIS

KEY WORDS:

dysbiosis, phytochemicals, inflammation, gut microbiota, adiposity.

PAGES: 60
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Polyphenols ingestion by diet can set part of strategies in the obesity treatment through its antioxidants, anti-inflammatories, and gut microbiota modulation effects. These actions influence widely the organism including the intestine. In this sense, systematization of scientific data regarding the effects and mechanisms of action from polyphenols on the gut health is important to lead strategies to combat the obesity. The study objective is to gather evidence about the impact of polyphenolic compounds on intestinal health of experimental models with diet-induced obesity. The protocol was designed according to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P), approved by the International Prospective Register of Systematic Reviews (PROSPERO –CRD42021262445),and published at Medicine journal (DOI  10.1097/MD.0000000000028162). Based on the structuring of the research question “What are the effects of polyphenols on intestinal health in preclinical studies with diet-induced obesity?”, the eligibility criteria were defined according to the acronym PICOS (criteria for population, intervention, comparison, outcomes and types of studies). The development of the systematic review was outlined by the Preferred Reporting Items for Systematic Review and Metanalysis (PRISMA). Each step of the review was carried out independently by two researchers. Experimental studies in rats and mice with diet-induced obesity and treated with isolated polyphenols were included, retrieved through the formulation of search strategies applied in the following scientific study databases: PubMed, Science Direct, Scopus, Web of Science and EMBASE. The methodological quality of each selected study was evaluated using the Systematic Review Center for Laboratory Animal Experimentation (SYRCLE) tool by two independent evaluators. There was a high degree of agreement between the evaluators (Kappa coefficient >0.85). Initially, 1415 publications were retrieved. After  reading the title and abstract, following the inclusion and exclusion criteria, 183 studies remained. Data were extracted and analyzed from the articles included regarding intestinal health (modulation of the microbiota, and other parameters, such as production of short-chain fatty acids, analysis of tight junctions of the intestinal barrier, endotoxemia, morphology and histology and expression of cytotoxins of intestinal tissue). After reading the complete works, 20 articles were included, the last two being added by manual search in the references of the included articles. The SYRCLE assessment showed scores between 4.5 - 7.0 (maximum 10). Data extraction and analysis showed clear changes in the intestinal microbiota, such as an increase in alpha and beta diversities (46.7% and 68.8% of the studies that performed the analysis, respectively), reduction of the F:B ratio (80% of the studies that performed the analysis), and population changes at phylum, family and genera levels, through treatment with different phenolic compounds. It was also observed in all studies the increase in the total production of short-chain fatty acids, the expression of tight junctions in the epithelial tissue of the intestinal barrier, improvement in the morphology and histology of the intestinal tissue, reduction of LPS (endotoxemia) in 88.9% of the studies that performed the analysis, reduction of inflammatory cytokines, mainly TNF-α and IL-6. Therefore, it is concluded that the administration of polyphenols in animal models induced obesity can help in the treatment of obesity by modulating the gut microbiota and improving parameters related to intestinal health.

COMMITTEE MEMBERS:
Externa à Instituição - ÂNGELA GIOVANA BATISTA - UFJF
Externa ao Programa - 2665457 - GIDYENNE CHRISTINE BANDEIRA SILVA DE MEDEIROS - nullExterna ao Programa - 2495705 - GRASIELA PIUVEZAM - nullPresidente - 1971809 - JULIANA KELLY DA SILVA MAIA