Banca de QUALIFICAÇÃO: KARINA ZAIRA SILVA MARINHO COSTA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : KARINA ZAIRA SILVA MARINHO COSTA
DATE: 11/10/2022
TIME: 14:00
LOCAL: Sessão pública realizada por videoconferência
TITLE:

Differential expression of microrna and its relationship to micronutrient concentration and the inflammatory response in women with hyperglycemia

KEY WORDS:

microRNA, hyperglycemia, inflammation, micronutrients.

PAGES: 50
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Hyperglycemia is important in the development and maintenance of chronic inflammation and oxidative stress in overweight individuals and is a risk factor for cardiovascular disease, metabolic syndrome and diabetic complications. Nutrients with important anti-inflammatory and antioxidant action can interact with the genome, altering its expression and modifying the phenotype, including the risk for chronic non-communicable diseases (NCDs). This interaction allows the modulation of microRNA (miRNA), which are important for the regulation of several molecular mechanisms. Therefore, the objective of this work was to identify differentially expressed microRNA in women with hyperglycemia and its association with inflammatory biomarkers and essential micronutrients. This is a comparative cross-sectional study derived from a study with 72 women between 18 and 60 years old and BMI ≥ 25.0kg/m². Analyzes were performed to determine the plasma concentration of zinc, selenium and manganese, miRNA, biochemical and inflammatory profile, in addition to anthropometric evaluation. Most participants did not have hyperglycemia (68.1%) and the median age of hyperglycemic women was 46.0 (44.0; 50.0) and of normoglycemic women was 39.0 (31.0; 45. 0). miR-29a-3p and miR-548c-5p were up-regulated in hyperglycemic women. Furthermore, a positive correlation was observed between miR-29a-3p and leptin concentration (rho=0.50; p=0.047), and a negative correlation between miR-548c-5p and IL-10 concentration (rho= -0.682; p=0.021) and LDL-c (rho=-0.673; p=0.023) in hyperglycemic women. There was also a positive correlation between miR-548c-5p and HDL-c (rho=0.462; p=0.035) in normoglycemic women. Differences in zinc, selenium and manganese concentrations were not identified between women with and without hyperglycemia, and no correlations were found between these minerals and up-regulated microRNAs. Enrichment analysis of the target gene pathway identified that these miRNAs are potentially related to glycemic metabolism, the pathophysiology of hyperglycemia and diabetes or disease complications. Therefore, it is understood that these miRNAs have the potential to be used as biomarkers of hyperglycemia, especially miR-29a-3p, in addition to being involved in the activation of inflammatory pathways in these patients. Furthermore, the results of this study suggest that the micronutrients zinc, selenium and manganese are not related to the modulation of the expression of these miRNAs.

 

COMMITTEE MEMBERS:
Presidente - 3218336 - BRUNA ZAVARIZE REIS
Externo à Instituição - ERNESTO VARGAS MENDEZ - UCR
Interna - 2306763 - KARINE CAVALCANTI MAURICIO DE SENA EVANGELISTA
Externo à Instituição - MARIA ADERUZA HORST - UFG