Banca de DEFESA: NEYNA SANTOS MORAIS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : NEYNA SANTOS MORAIS
DATE: 24/09/2021
TIME: 09:00
LOCAL: Sessão pública realizada por meio de vídeo conferência
TITLE:

EVALUATION OF THE SAFETY AND BIOACTIVE POTENTIAL OF BURITI OIL (Mauritia flexuosa L. f.) NANOENCAPUSLED IN PORCINE GELATINE

 

KEY WORDS:

Nanoencapsulation; Cytotoxicity, Reducing Power; Total Antioxidant Capacity, Gentamycin; Norfloxacin

PAGES: 66
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Buriti oil extracted from the fruit of the buriti tree (Mauritia flexuosa L.f.) and is rich in carotenoids, especially β-carotene in addiction to tocopherols, unsaturated fatty acids, other nutrients and bioactive compounds that have antioxidant characteristics. The sensitivity to factors such as light, heat, humidity, pH and oxygen that buriti oil and bioactive compounds have and the lipophilic nature of the oil justify the use of nanoencapsulation to preserve and enhance the oil's bioactive properties. The aim of the present study was, evaluate the cytotoxicity, antioxidant potential, antimicrobial effect on the modulation of the antibiotic activity of porcine gelatin nanoparticles containing buriti oil (OGS)The nanoformulation was obtained through the O/W emulsification technique, using porcine gelatin as an encapsulating agent and Tween 20 as a surfactant. Characterization was performed by Scanning Electron Microscopy (SEM), Laser Diffraction and Fourier Transform Infrared Spectroscopy (FTIR), in addition to evaluating the encapsulation efficiency (%). The cytotoxicity analysis was performed on CHO cells (Chinese Hamster Ovary Cells) using the MTT test ((3-(4,5-Dimethylthiazol-2-yl) -2,5-Diphenyltetrazolium Bromide). The determination of the antioxidant potential of free buriti oil and OGS was performed by different in vitro methods, such as total antioxidant capacity (TAC), Reducing Power and scavenging to ABTS radical. The evaluation of the modulating antimicrobial activity was performed by determining the minimum inhibitory concentration (MIC), using Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacteria and two antibiotics, gentamicin and norflaxacillin. The SEM showed spherical particles with smooth, uniform surfaces and no cracks or depressions. The particle size and polydispersion index (PI) obtained by means of Dynamic Light Scattering (DLS) were 72 nm e 0,371nm, respectivamente respectively. The FTIR showed chemical interactions in OGS due to displacement and formation of new vibrational bands, indicating the interaction of oil with porcine gelatin and confirming encapsulation. The encapsulation efficiency obtained was 89.56%. The cytotoxicity analysis showed that crude buriti oil and OGS did not show cytotoxic effect on CHO cells. The results of the antioxidant activity showed that for both the TAC test and the reducing power, OGS presented greater antioxidant activity when compared to free buriti oil (p < 0.05). For the modulating antimicrobial activity, it was observed that the combination of antibiotics, norfloxacillin and gentamicin, with free buriti oil and OGS was more efficient to inhibit the action of E. coli and P. aeruginosa compared to isolated antibiotics (p < 0 .05). Regarding the inhibition of S. aureus, OGS stood out in combination with norfloxacillin, reducing MIC by 50%. Thus, it was observed that nanoencapsulation increased the antioxidant potential and antimicrobial activity of buriti oil, which can be explained by the protection given to bioactive compounds with these properties.

BANKING MEMBERS:
Presidente - 2378605 - CRISTIANE FERNANDES DE ASSIS
Externa à Instituição - MARIA GIOVANA BINDER PAGNONCELLI
Externa ao Programa - 011.694.104-99 - NATHALIA KELLY DE ARAUJO - IFRN
Externa ao Programa - 2275877 - THAIS SOUZA PASSOS