Banca de DEFESA: CLEIDIJANE ANTERO DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CLEIDIJANE ANTERO DOS SANTOS
DATE: 01/06/2020
TIME: 09:00
LOCAL: Defesa realizada de forma virtual pela plataforma Google Meet
TITLE:

ASTAXANTIN EXTRACTION FROM SHRIMP (Litopenaeus vannamei) FLOUR USING EUTHETIC SOLVENTS FOR FOOD MATRIX APPLICATION

KEY WORDS:

high performance liquid chromatography, ultrasound, antimicrobial, carotenoids

PAGES: 60
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Astaxanthin is the main carotenoid pigment found in shrimp residues, and has numerous beneficial properties for human health. The extraction of astaxanthin can be done using different organic solvents, however is being replaced by “Deep Eutectic Solvents” (DES) it are considered an efficient alternative in the extraction of polar and nonpolar compounds. The objective of this work was propose a strategy to increase the yield of the extraction of astaxanthin from shrimp (Litopenaeus vannamei) flour using ethanol as an extracting phase and DES (glycerol-choline chloride) and choline chloride as adjuvants. In addition, the quantification of astaxanthin was performed  by high performance liquid chromatography. The acid fatty perfil was analizaded by Gasosa Cromatografy and the bioactive properties of the extracts were evaluated antioxidant activities (by 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid - ABTS) and antimicrobial. The results showed that the use of ultrasound as the variable that most interferes with the extraction of astaxanthin. Under conditions assisted by ultrasound, the addition of 5% (m / m) DES resulted in the highest release of astaxanthin (32.71 µg / g of flour) in short incubation times when compared to extractions involving only ethanol (maximum concentration of 30,99 µg / g of flour after 30 min). The concentration of 2.68 µg / mL of the shrimp flour extract was able to inhibit 50% of the ABTS radical. The antibacterial activity showed a significant inhibitory effect at the minimum concentration of 1024µg / mL, whose values were statistically similar to those obtained from synthetic astaxanthin. In short, the use of DES and ultrasound has proved to be a potential alternative for recovery of astaxanthin from shrimp waste with potential bioactive properties. 

BANKING MEMBERS:
Externo à Instituição - CARLOS EDUARDO DE ARAÚJO PADILHA - UFRN
Presidente - 2378605 - CRISTIANE FERNANDES DE ASSIS
Interna - 1971809 - JULIANA KELLY DA SILVA MAIA
Externa à Instituição - NATHALIA KELLY DE ARAUJO - IFRN