Banca de DEFESA: ADILA DANIELLY DE SOUZA COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ADILA DANIELLY DE SOUZA COSTA
DATE: 29/05/2020
TIME: 14:30
LOCAL: Defesa realizada de forma virtual pela plataforma Google Meet
TITLE:

INFLAMMATORY PROFILE OF INDIVIDUALS WITH DUCHENNE MUSCULAR DYSTROPHY: CORRELATIONS BETWEEN CLINICAL AND NUTRITIONAL PARAMETERS

KEY WORDS:

cytokines, interleukin-1, interleukin-6, tumor necrosis factor-alpha, nutritional status, body composition.

PAGES: 76
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Background & aims: Duchenne Muscular Dystrophy (DMD) is the most frequent dystrophy. Chronic inflammation appears as an important feature of DMD pathophysiology and progression, with cytokines being biomarkers of systemic inflammation and indicative of muscle damage in DMD. Objective: To identify the inflammatory profile of individuals with DMD and to evaluate associations between clinical and nutritional variables. Methods: a prospective study was conducted with data collection between January 2018 and June 2019, in the city of Natal, Northeastern Brazil. Male patients with a confirmed diagnosis of DMD were included. Sociodemographic and illness time data were obtained by interview; the use of medications and supplements was assessed based on information from the patient's medical record. The nutritional status was assessed according to the body mass index (BMI) and the body composition determined with the aid of dual emission x-ray densitometry (DXA). Plasma cytokines IL-1β, IL-6, and TNF-�, were measured using the ELISA test. The relationship between illness time, nutritional status, body composition, and cytokines was assessed by the Wilcoxon test. Spearman's correlation coefficient and multiple linear regressions were used to establish the relationship between the time of the first signs of DMD, fat mass (FM), and cytokines. Results: Forty-four individuals aged 4.3 to 24.2 years were evaluated. At the time of data collection, individuals had been for 7.8 (4.7–10.5) years since the onset of the first signs of the disease. Concerning nutritional status, 18.2% of the individuals presented marked thinness, thinness or underweight, 40.9% were eutrophic, and 40.9% presented the risk of overweight, overweight, or obesity. The mean %FM was of 33,9 (15,9–50,2) %. The %FM increased (p <0.001) since the beginning of the first signs of DMD. The illness time was positively correlated with IL6 (ρ = 0.371 p = 0.013). BMI/Age correlated negatively with IL-1β and TNF-α (ρ = - 0.357 p = 0.030 and ρ = -0.353 p = 0.032), while the %FM showed a negative relationship with TNF-α (ρ = -0.435 p = 0.005). The regression models demonstrated lower z-scores of BMI/A associated with higher values of IL-1β (β = -0,498 p = 0,003) and IL-6 (β = -0.640 p = 0.004), higher %FM associated with higher IL-6 (β = 0.582 p = 0.007) and lower TNF-α (β = -0.415 p = 0.015). Conclusion: A persistent inflammatory profile was observed in the patients evaluated. The illness time was not a predictor for the values of the evaluated cytokines. Lower values of BMI/A and higher values of %FM were predictors of higher concentrations of IL-6, while lower values of %FM were associated with higher production of TNF-α. The data suggest that maintaining adequate nutritional status and body composition are important for determining the inflammation presented by individuals with DMD.

BANKING MEMBERS:
Presidente - 2646462 - BRUNA LEAL LIMA MACIEL
Externa à Instituição - FABIANA MARIA COIMBRA DE CARVALHO
Interna - 2306763 - KARINE CAVALCANTI MAURICIO DE SENA EVANGELISTA