Banca de DEFESA: GRAZIELLE LOUISE RIBEIRO DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GRAZIELLE LOUISE RIBEIRO DE OLIVEIRA
DATE: 24/03/2020
TIME: 08:00
LOCAL: Sala de aula 07 do Departamento de Nutrição
TITLE:

Evaluation of the antioxidant potential and stability of carotenoid-rich extract of Cantaloupe melon nanoencapsulated for food application

KEY WORDS:

β-carotene. Nanoencapsulation. Antioxidant activity.

PAGES: 60
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Nanoencapsulation presents itself as an alternative to promote increased solubility, stability, potentiate, and preserve the antioxidant action of carotenoids. Based on this, the present study aimed to evaluate the antioxidant potential and stability of the carotenoid-rich extract of Cantaloupe melon nanoencapsulated in gelatin, aiming at the application in food. Nanoformulation (EPG) was obtained by oil in water (O / W) emulsification, using crude extract rich in carotenoids (CE), porcine gelatin (encapsulating agent), and Tween 20 (surfactant). The particles obtained were characterized by Scanning Electron Microscopy (SEM), Laser Diffraction, and Fourier Transform Infrared Spectroscopy (FTIR), in addition to determining the incorporation efficiency. The following groups were evaluated for antioxidant activity using antioxidant tests using 2,2-diphenyl-2-picrylhydrazyl (DPPH •) and 2,2´-azino-bis- (3-ethylbenzoatiazolin) -6-sulfonic acid ( ABTS •): (1) CE (0.05 mg / mL); (2) commercial standard β-carotene (0.009 mg / mL); (3) nanoparticles without CE (4.00 mg / mL); (4) EPG (4.05 mg / ml) and; (4) CE extracted from the nanoparticles (0.05 mg / mL). All groups were evaluated for β-carotene retention by UHPLC, and stability of antioxidant activity by ABTS, being subjected to food storage conditions at room temperature and refrigeration (25 ° C and 5 ° C, respectively) with and without the presence of light (1400 lux), for 45 days. The results of the characterization demonstrated the presence of spherical particles with a smooth surface and medium diameter in the nanometer scale [90.9 (7.20) nm and 0.56 (0.08)]. The FTIR showed chemical interactions between the crude extract and the swine gelatin, confirming the encapsulation. The following results were obtained for oxidation inhibition (%) by ABTS and DPPH, respectively, for the groups evaluated: 1) CE: 22.24 (1.93)% and 18.76 (0.95)%; (2) standard β-carotene: 10.70 (1.55)% and 30.57 (0.54)%; (3) nanoparticles without CE: 0.0 (0.0)% and 0.0 (0.0)%; (4) EPG: 0.0 (0.0)% and 0.0 (0.0)%; (4) CE extracted from nanoparticles: 34.95 (3.04)% and 29.96 (0.25)%. Therefore, nanoencapsulation promoted an increase in the range of 57-59% in the antioxidant activity of the crude extract. The stability study showed that after 45 days of storage, CE showed low retention of β-carotene, in the range of 0 to 43.6%, with the lowest percentages related to storage conditions at room temperature under light ( 0.00%) and in the dark (11.7%) and, the highest retention associated with the condition under refrigeration and dark (43.6%). As a result, it was observed that this low stability of carotenoids, consequently, had a negative impact on antioxidant activity, which was completely lost under conditions of light and dark at room temperature (0.0%), maintaining only 5.9 - 13.2 % of activity under storage conditions under refrigeration in light and dark, respectively. For CE nanoencapsulated, extracted from EPG, at the end of 45 days, retention of 100% β-carotene was observed in dark conditions at room temperature and under refrigeration (p> 0.05). For the conditions of exposure to light, at room temperature, there was a total loss of β-carotene content (0.0%) and, under refrigeration, retention of 86%. Therefore, it is observed that nanoencapsulation promoted an increase in carotenoid stability under refrigeration and at room temperature in the absence of light. Besides, it is noted the excellent result obtained under refrigeration in the presence of light, showing the enhancement of the stability of β-carotene, as in this condition, the crude extract not encapsulated only showed retention of 18.5% of the carotenoid. Regarding antioxidant activity, preservation was found in the range of 70 - 50% after 45 days, with the highest percentages also associated with storage conditions in the dark at room temperature and under refrigeration. Therefore, the study demonstrated that through Nanotechnology, it was possible to enhance and promote the increased stability of Cantaloupe melon carotenoids and, consequently, the preservation of biological activity directly associated with several benefits to human health. Thus, increasing the potential application of natural dyes with bioactive potential, given the preservation of antioxidant activity, in industrialized foods with an emphasis on those stored under refrigeration with or without exposure to light and, at room temperature, in packaging that serve as a barrier to the passage of light.

BANKING MEMBERS:
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Interno - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externa ao Programa - 2275877 - THAIS SOUZA PASSOS
Externa à Instituição - ISABEL RODRIGUEZ AMADO