Banca de DEFESA: ISAIANE MEDEIROS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ISAIANE MEDEIROS
DATE: 25/03/2020
TIME: 08:30
LOCAL: Auditório do Departamento de Infra Estrutura - UFRN
TITLE:

EVALUATION OF THE SAFETY AND BIOACTIVE POTENTIAL OF GELATIN NANOPARTICLES LOADED WITH CAROTENOIDS EXTRACT FROM CANTALOUPE MELON (Cucumis melo L.) ADMINISTERED IN WISTAR RATS

KEY WORDS:

β-carotene. Curcubitaceae. Nanotechnology. Toxicity

PAGES: 70
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Natural pigments have numerous bioactive properties, especially carotenoids that have powerful anti-inflammatory action on the human body. However, carotenoids are unstable in the presence of light, acids, oxygen, and heat, which can lead to the loss of antioxidant effects related to the consumption of these molecules. Nanoencapsulation appears as a strategy to preserve or enhance the anti-inflammatory action, in addition to increasing the solubility and bioavailability of carotenoids. Therefore, it is necessary to evaluate the safety of nanoparticles for future clinical applications. Thus, the objective of this study was to evaluate the safety and bioactivity of nanoparticles based on crude Cantaloupe melon extract rich in carotenoids and porcine gelatin in Wistar rats. Encapsulation was performed by oil/water emulsification followed by lyophilization, and porcine gelatin was used as an encapsulating agent and Tween 20 as a surfactant. Nanoformulation (EPG) was characterized by different physical and chemical analyzes and evaluated for incorporation efficiency (EI). Toxicity was investigated through signs of general toxicity and food consumption in male Wistar rats (n = 10) fed with a high glycemic index and glycemic load (HGLI) diet treated with EPG administered by gavage (50 mg/Kg), and crude extract rich in carotenoid (12.5 mg/kg) for ten days, using as controls a group without treatment and another treated with a standard diet. Blood toxicity (blood count, glucose, triglycerides, total cholesterol, liver, and kidney function), morphology and histopathology of the liver, small intestine, kidneys, stomach, and spleen, were evaluated. The characterization of EGS pointed to the presence of spherical particles with a smooth surface, an average diameter of 74 (12.7) nm, a polydispersion index of 0.53 (0.12), and EI of 98% (1.78). For signs of general toxicity, there was no significant difference (p> 0.05) between the initial and final weight of the rats in each evaluated group. There was a significant increase (p <0.05) in food consumption only in the group without treatment. Through the biochemical parameters analyzed, low toxicity of EGS was found. The results obtained for the determination of the relative weight of the livers, spleen, small intestine, kidneys, and stomach showed that there was no significant difference (p> 0.05) between the studied groups. The morphological and histopathological analyses of the liver showed acute hepatitis in the animals of all the groups evaluated, and those treated with EB and EGS showed tissue repair. For small intestine, chronic enteritis was observed in all animals, with a considerable improvement in the villi and intestinal glands of animals treated with EGS. The stomach, spleen, and kidneys do not present a histopathological alteration. The results suggest the safe application of EGS and a bioactive effect, possibly related to the anti-inflammatory potential, showing the safety regarding the use in future clinical studies, aiming to investigate the efficacy in the control of inflammatory processes.

BANKING MEMBERS:
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externo ao Programa - 1076490 - FERNANDO VAGNER LOBO LADD
Externa à Instituição - JAILANE DE SOUZA AQUINO - UFPB
Externa ao Programa - 2275877 - THAIS SOUZA PASSOS