Banca de QUALIFICAÇÃO: CLEIDIJANE ANTERO DOS SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : CLEIDIJANE ANTERO DOS SANTOS
DATA : 27/09/2019
HORA: 09:00
LOCAL: Sala de aula 7 do Departamento de Nutrição - DNUT/UFRN
TÍTULO:

ASTAXANTHIN EXTRACTION FROM THE SHRIMP (Litopenaeus vannamei) WASTE USING DEEP EUTECTIC SOLVENTS  FOR FOOD MATRIX APPLICATION

PALAVRAS-CHAVES:

DES, DPPH, ultrasonic, antimicrobial

PÁGINAS: 60
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Astaxanthin is the main carotenoid pigment found in residues (head and cephalothorax) of shrimp in conjunction with proteins (carotenoproteins). The predominant carotenoid is astaxanthin which has antioxidant, anti-inflammatory, immunomodulatory, anticancer, antidiabetic activity, prevents cardiovascular diseases, and also acts to prevent cataract. The extraction of astaxanthin can be done using different organic solvents, but their use is being replaced by more ecological forms. The use of deep eutectic solvents (DES) has been increasing because they are considered an efficient alternative in the extraction of polar and non polar compounds. The objective of this work was to propose a form of astaxanthin extraction using DES under different conditions, to obtain a higher concentration of astaxanthin and to realize studies of antioxidant and antimicrobial activity of astaxanthin obtained compared to synthetic astaxanthin. The raw material was the waste (cephalothorax) of shrimp L. vannamei obtained from a shrimp processing company in RN/Brazil. Different variables were used for extraction: time, solvent and ultrasound. Astaxanthin was quantified by spectrophotometer and High Performance Liquid Chromatography (HPLC). For antimicrobial activity the microorganisms Staphylococcus aureus and Enterococcus faecalis were used and for the antioxidant activity the DPPH test was performed. The results show ultrasound as the variable that most interferes with astaxanthin extraction. From this result three conditions were chosen using ultrasound for the astaxanthin concentration to be analyzed by HPLC and two of them obtained similar values, using ethanol for 30 minutes (30.99 32µg / g flour) and using 5% DES per 10 minutes (32µg / g flour). For the antimicrobial and antioxidant activity assays the sample chosen was the one that used the DES (5%) because the extraction time was shorter. Inhibition of 62.73% and 69.06% was observed for extracted astaxanthin and 66.29 and 68.09% for synthetic astaxanthin for growth of the microorganisms Staphylococcus aureus and Enterococcus faecalis, respectively in the concentration of 20µg/mL. For the DPPH test, extracted astaxanthin and synthetic astaxanthin were used, both at 15 µg/mL and the results were: 30.91 and 30.15% inhibition of DPPH radical, respectively. According to the results it can be concluded that astaxanthin extraction using DES is efficient and that the solvent used in the extraction does not alter the properties of astaxanthin compared to synthetic astaxanthin.

MEMBROS DA BANCA:
Presidente - 2378605 - CRISTIANE FERNANDES DE ASSIS
Externa à Instituição - NATHALIA KELLY DE ARAUJO
Externo à Instituição - PEDRO FERREIRA DE SOUZA FILHO