Banca de QUALIFICAÇÃO: LÍDIA LEONIZE RODRIGUES MATIAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : LÍDIA LEONIZE RODRIGUES MATIAS
DATA : 28/02/2019
HORA: 13:00
LOCAL: Sala de Reuniões do Departamento de Saúde Coletiva - DSC/UFRN
TÍTULO:

EFFECT OF TRYPSIN INHIBITOR ISOLATED FROM TAMARIND SEEDS ENCAPSULATED ON BIOCHEMICAL PARAMETERS OF OVERFED WISTAR RATS

PALAVRAS-CHAVES:

Trypsin Inhibitor; Encapsulation; Overfeeding, Stability; Digestibility; Fasting glucose, Lipid profile.

PÁGINAS: 70
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Many studies have shown the benefits of protease inhibitors, especially of Trypsin Inhibitor Isolated from tamarind (Tamarindus indica L.) seeds (TTI), which acts to improve several biochemical parameters in Wistar rats. Thus, there is the search for alternatives to intensify the effect of the TTI, being an option the encapsulation, which allows a controlled release and potentialized effect of the encapsulated material. Therefore, this study aimed to evaluate the encapsulated TTI (ECW) to antitrypsin activity at acid pH, and also in vitro against the physiological conditions of the gastrointestinal tract. In addition to determining the effect on biochemical parameters (glucose, insulin and lipid profile) in overfed Wistar rats. TTI was obtained by protein fractionation using ammonium sulfate, followed by Affinity Chromatography in Trypsin-Sepharose. For the encapsulation by nanoprecipitation, an aqueous solution containing TTI, whey protein isolate, purified chitosan (1: 2: 2 w/w/w) and Tween 80 was prepared. Absolute ethanol was used to promote the precipitation of the particles under the action of a High-performance dispersing. ECW was dehydrated in ethanol, filtered, lyophilized and characterized by various physical and chemical methods. Subsequently, the particles were evaluated in vitro for antitrypsin activity at acidic pH, using several steps of ultrafiltration in Amicon® 100 K. The stability to the conditions of the gastrointestinal tract was evaluated in vitro in three phases: oral, gastric and intestinal, and monitored by SDS-PAGE gel protein profile and by High-Performance Liquid Chromatography (HPLC). Besides, ECW was evaluated in vivo by oral gavage (12.5 mg/kg) in overfed Wistar rats with a high glycemic index diet and high glycemic load (HGLI diet). The results showed that, at acidic pH, the ECW interacts chemically with the encapsulating agents in all the evaluated steps, indicating greater preservation of the antitrypsin activity in the retained particles in the membrane [68.10% (0.70)] than the isolated ITT [8.30% (2.60)]. The ECW was not susceptible to gastric enzymes, maintaining its antitrypsin activity at 100%, while the isolated ITT was partially hydrolyzed, completely missing the antitrypsin activity and protein peak characteristic of TTI observed by HPLC. Against intestinal enzymes, ECW and TTI were susceptible to hydrolysis, as confirmed by HPLC. The ECW administered in overfed Wistar rats, significantly reduced glucose and increased HDL cholesterol (p <0.05). The encapsulation technique promoted greater protection for TTI and useful improvement of the biochemical parameters related to the onset of chronic non-communicable diseases, using a concentration of inhibitor ten times lower than that administered in vivo studies with TTI isolated.

MEMBROS DA BANCA:
Interno - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externa à Instituição - ISABEL RODRÍGUEZ AMADO
Externa ao Programa - 2275877 - THAIS SOUZA PASSOS