Banca de DEFESA: ANNA BEATRIZ SANTANA LUZ

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANNA BEATRIZ SANTANA LUZ
DATA : 14/05/2018
HORA: 14:00
LOCAL: Auditório da Superintendência de Infraestrutura - SIN/UFRN
TÍTULO:

Adipocytes and intestinal epithelium dysfunctions linking obesity to inflammation induced by High Glycemic Index Pellet-diet in Wistar rats.

PALAVRAS-CHAVES:

Adiposity; Animal feed; Dietary sucrose; TNF-α.

PÁGINAS: 63
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Research to combat metabolic, body and biochemical changes has focused on the use of diets similar to the dietary pattern of the current population to induce disorders in animals. This study investigated the inflammatory effect of a pellet-diet with high glycemic index and load (HGLI) on the histological organization of adipocytes, intestinal epithelium and fat in liver and pancreas in Wistar rats. Two groups of adult animals (n = 5) received for 17 weeks: (1) HGLI Diet or (2) Standard Diet (Labina®). Microscopic analysis was performed with a diagnostic reading of the slides and an emphasis on the histological organization of visceral adipose tissue and the jejunum, as well as the investigation of the presence of adipocytes in the liver and pancreas. In addition, the investigation of volume density, total volume and cross-sectional area of adipocytes present in the visceral adipose tissue was performed. Furthermore, the calculation of the adiposity index was obtained considering as total visceral fat the sum of the perirenal, retroperitoneal and epididymal adipose tissue compartments. For gene expression, RT-qPCR was performed on the following genes: TNF-α (LOC103694, Rn01525859_g1) and GAPDH (Rn01775763_g1). Immunohistochemistry was performed using the rabbit polyclonal primary antibody: anti-TNF-α (1: 100). The intensity of TNF-α was measured semi-quantitatively and automatically. The images were scored according to a note, which consisted of the following variation: negative (0), low positive (+1), positive (+2) and high positive (+3). Serum was used for TNF-α. The HGLI diet induced hypertrophy of adipocytes with adipocyte volume density equal to 97.0%, cross-sectional area equivalent to 1387 μm² and a total volume of 6.97 cm³ an elevation of 8%, 25%, and 58%, respectively. Furthermore, the HGLI diet increased liver and pancreatic fat deposition, caused intestinal villi disorganization and increased TNF-α gene expression (p = 0.014) with a positive immunostaining (+2) in visceral adipose tissue and high plasma TNF-α in comparison to standard diet. The HGLI diet induced inflammation in adipocytes and intestinal epithelium of Wistar rats, dysfunctions are commonly caused by pellet-diets with high fat or fructose-rich beverages.

MEMBROS DA BANCA:
Externo à Instituição - ALEXANDRE COELHO SERQUIZ - UNI-RN
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Interno - 1879430 - ANA PAULA TRUSSARDI FAYH