Banca de DEFESA: JALUZA LUANA CARVALHO DE QUEIROZ

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JALUZA LUANA CARVALHO DE QUEIROZ
DATA : 11/05/2018
HORA: 14:00
LOCAL: Auditório da Superintendência de Infraestrutura - SIN/UFRN
TÍTULO:

Chitosan-whey protein nanoparticles improve encapsulation efficiency and stability of a trypsin inhibitor isolated from Tamarindus indica L.

PALAVRAS-CHAVES:

Encapsulation; Anti-triptytic activity; Stability In vitro.

PÁGINAS: 65
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Studies have shown that the trypsin inhibitor isolated from tamarind (Tamarindus indica L.) (TTI) seeds have a satietogenic and anti-inflammatory action in an experimental model. Based on this and aiming to increase the efficiency and stability of the antitríptic activity, the present study investigated the isolated and conjugated effect of chitosan and isolated milk protein in the incorporation of TTI. The inhibitor was extracted from tamarind seeds and isolated by affinity chromatography on Trypsin-Sepharose. For the encapsulation, the nanoprecipitation technique was used in an organic solvent (ethanol), and the encapsulating agents evaluated were chitosan, whey protein isolate and a combination of both, respectively, in TTI: encapsulating agent ratio of 1: 4, 1: 4 and 1: 2: 2 w/w. The obtained particles were evaluated for the efficiency of incorporation, characterized by different physical and chemical methods, determination of the amount (mg) of inhibitor that reduces the activity of the trypsin by 50% (IC 50) and stability at different temperatures (40, 60, 80 and 100°C). Regarding the efficiency of incorporation, there was no statistical difference between the evaluated formulations (p> 0.05). SEM of the encapsulates showed the formation of spherical particles with a smooth surface, with the exception of that based on chitosan, in which spherical particles were observed, with surfaces filled with depressions. FTIR spectra provided indicative of ITT encapsulation with the three wall materials studied (purified chitosan, whey protein isolate, and chitosan-whey protein isolate). The diffractograms indicated that all the encapsulates presented amorphous structure. The encapsulation mainly with chitosan and the combination of wall materials promoted a reduction in IC50, respectively, equal to 0.04 mg and 0.05 mg, compared to 0.21 mg of ITT alone, which was proven to be inhibitory action, considering that the particles isolates showed no antitryptic activity. Regarding stability, only chitosan-whey protein isolate preserved the antitrytry activity up to 80°C. Thus, it can be concluded that the combination of encapsulating agents was an extremely important strategy to enhance the function and stability of the TTI.

MEMBROS DA BANCA:
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externo ao Programa - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externo à Instituição - NORBERTO DE KASSIO VIEIRA MONTEIRO - UFC
Externo ao Programa - 2275877 - THAIS SOUZA PASSOS