Banca de DEFESA: ANNY KAROLINY DE OLIVEIRA CAVALCANTI MEDEIROS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANNY KAROLINY DE OLIVEIRA CAVALCANTI MEDEIROS
DATA : 04/05/2018
HORA: 14:00
LOCAL: Auditório da Superintendência de Infraestrutura - SIN/UFRN.
TÍTULO:

Increasing solubility and stability in yogurt of cnantaloupe melon carotenoids (Cucumis melo L. reticulatus Naud) nanoencapsulated in gelatin by double emulsion W/O/W.

PALAVRAS-CHAVES:

Natural dye; Whey proteins; Porcine gelatin; Nanoparticles; Colorimetry.

PÁGINAS: 80
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

The objective of the study was produced and characterize the carotenoid-rich extract of Cantaloupe melon (Cucumis melo L. reticulatus Naud.) Encapsulated in porcine gelatine (EPG) and whey protein concentrate (EWPC) and isolate (EWPI), evaluate the solubility in water, and the color stability in yogurt. The determination of total carotenoids by UV-visible spectrophotometry and β-carotene by High Performance Liquid Chromatography (HPLC) was performed. The encapsulation was performed by double emulsion W/O/W. The particles were characterized by Dynamic Light Diffusion, Zeta Potential, Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), Fourier Transform Infrared Spectrophotometry (FTIR), and evaluated for incorporation efficiency, solubility in water, and stability in colorimetric yogurt (CIElab). The HPLC indicated 40 μg (0.02) of β-carotene/g of melon pulp Cantaloupe. The averages of particle size and polydispersity index were, respectively, 59.3 (2.6) nm and 0.377; 161.0 (27.3) nm and 0.614 and 123.3 (37.4) nm and 0.537 for EPG, EWPI, and EWPC. The results for the Zeta Potential were 21.2 (0.10) mV, -10.8 (0.55) mV and -14.2 (0.25) mV, respectively, for the EPG, EWPI and EWPC. SEM of the EPG showed particles with a smooth surface and of homogeneous sizes. The EWPI presented a smooth surface, but with many agglomerates and few particles on the nanoscale. For EWPC, smooth particles with no cracking and heterogeneous sizes were observed. All the obtained diffractograms revealed amorphous structures. The FTIR showed mainly for EPG and EWPC new bands not observed in the isolated materials. The incorporation efficiencies obtained for EPG, EWPC and EWPI were, respectively, 90% (7.39), 77% (8.65) and, 58.5% (0.71), without significant difference (p> 0,05) only between EPG and EWPC. In the solubility test, except for the crude extract of carotenoids and the EWPC (p> 0.05), all groups showed a statistically significant difference (p <0.05), with determined concentrations equal to 0.025 mg/ml (0.003) and 0.011mg/ml (0.0009), respectively, for EWPC and EWPI, and 0.073mg/ml (0.007) for EPG. This was three times higher than in the crude extract [0.0263mg / ml (0.003)] and, therefore, used in the stability study. In this evaluation, when the crude extract was used in yogurt (YCE), the a* index only showed no significant difference between the 0 and 15 days time (p> 0.05), presenting a significant difference (p <0.05) between the other evaluated times (30, 45 and 60 days) in comparison with time 0, indicating alteration of the tonality of the green coloration. For the b* index, there was a significant difference (p <0.05) between all evaluated times when compared with time 0, showing loss of yellow coloration. However, the yogurt added of EPG yogurt did not present a significant difference (p> 0.05) for any of the indices evaluated. Therefore, the encapsulation with porcine gelatin by double emulsion technique is a promising strategy for the encapsulation of carotenoids, aiming to improve water solubility and stability in the food matrix, thus increasing the potential of carotenoid application of Cantaloupe melon.

MEMBROS DA BANCA:
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externo ao Programa - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Externo à Instituição - KÁTIA GOMES DE LIMA ARAÚJO - UFF
Externo ao Programa - 2275877 - THAIS SOUZA PASSOS