Banca de QUALIFICAÇÃO: JALUZA LUANA CARVALHO DE QUEIROZ

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : JALUZA LUANA CARVALHO DE QUEIROZ
DATA : 28/02/2018
HORA: 14:00
LOCAL: Sala de aula A do Departamento de Nutrição - DNUT/UFRN
TÍTULO:

Chitosan-whey protein nanoparticles improve encapsulation efficiency and stability of trypsin inhibitor isolated from Tamarindus Indica (L.).

PALAVRAS-CHAVES:

Encapsulation; Anti-triptytic activity; Stability In vitro.

PÁGINAS: 70
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Studies have shown that the trypsin inhibitor isolated from tamarind (Tamarindus indica L.) (ITT) seeds have a satietogenic and anti-inflammatory action in an experimental model. Based on this and aiming to increase the efficiency and stability of the antitriptic activity, the present study investigated the isolated and conjugated effect of chitosan and milk isolated protein in ITT encapsulation. The inhibitor was extracted from the tamarind seeds and isolated by means of affinity chromatography on Trypsin-Sepharose. For the encapsulation, the nanoprecipitation technique was used in organic solvent, and the encapsulating agents evaluated were chitosan, milk protein isolated and mixture between them, respectively, in ITT ratios: 1: 4, 1: 4 and 1:2:2 w/w. The obtained particles were evaluated for the efficiency of encapsulation and, characterized by different physical and chemical methods. In addition, the concentration of inhibitor was determined which reduces trypsin activity by 50% (IC50). A stability study was carried out, using different pH and temperature conditions, in order to determine the influence of the encapsulating agent on the preservation of ITT antitriptic activity. The results showed that there was no significant difference (p <0.05) in relation to the encapsulation efficiency between the groups generated. The SEM showed the formation of spherical particles with a smooth surface, both in the ITT encapsulated with protein isolated from the milk and in the mixture, but with chitosan particles were observed without spherical shape and with surfaces full of depressions. The FTIR spectra of the materials provided indicative of ITT encapsulation with the three wall materials studied. The diffractograms indicated that the encapsulated presented amorphous structure. The IC 50 showed that in all materials there was a significant reduction in the ITT concentration required to inhibit trypsin activity by 50%, was 0.18 mg, 0.046 mg, and 0.065 mg, respectively, for the particles based on protein isolated from milk, chitosan, and mixtures. Thus, it can be concluded that the mixture of materials becomes an extremely important tool to improve the function of the inhibitor, protecting it against external actions and, guaranteeing better results in low concentrations.

MEMBROS DA BANCA:
Externo ao Programa - 1549705 - ADRIANA FERREIRA UCHOA
Externo ao Programa - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Externo ao Programa - 2275877 - THAIS SOUZA PASSOS