Banca de DEFESA: MYCHELLE KYTCHIA RODRIGUES NUNES DUARTE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MYCHELLE KYTCHIA RODRIGUES NUNES DUARTE
DATA : 06/12/2016
HORA: 14:00
LOCAL: Sala de aula 06 do Departamento de Nutrição - DNUT/UFRN.
TÍTULO:

Biomarkers for early detection of cardiovascular disease: relationship of biomolecules, nutritional status with extent of coronary lesion.

PALAVRAS-CHAVES:

Oleic acid, gene expression, cardiovascular disease, atherosclerosis, index Friesinger, ECHDC3, intake ingest, food consumption and dietary intake, visceral fat.

PÁGINAS: 108
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Introduction: Cardiovascular diseases (CVD)  is the leading cause of death worldwide, that can be triggered by alterations in the serum profile of fatty acids, by patterns of gene expression, changes in dietary intake, and alteration anthropometric. The early diagnosis of this pathophysiology is a challenge in translational medicine, since the methods used are expensive and / or invasive. Purpose: To identify biomarkers for the early diagnosis of cardiovascular diseases through the relationship of biomolecules (fatty acids and ECDHC3) and nutritional status (anthropometry, food consumption and dietary intake) with extensions of coronary lesion. Material and methods: The study was observational, with the sample collected for convenience in two casuistry in the Hemodynamics sector of the Onofre Lopes University Hospital (HUOL), 59 patients (n1, 2011-2013) and the second included 41 individuals ( N2, 2014-2015), both aged 30-74 years and who were undergoing coronary angiography for the first time. The extent of atherosclerotic lesions was determined by Friesinger index, the biochemical profile by semi-automated spectrometry; the concentration of fatty acids by gas chromatography; gene expression of ECHDC3 mRNA by real-time PCR; food consumption and dietary intake for two 24-hour dietary intake recalls and anthropometric parameters by the electric bioimpedance, measuring tape and stadiometer scale. Results: Patients were classified into groups: no lesion (n1 = 18 / n2 = 8), low lesions (n1 = 17 / n2 = 6), intermediate lesions (n1 = 17 / n2 = 15) and major lesion (n1= 7 / n2 = 12). In n1 was observed high serum concentrations of oleic acid and monounsaturated fatty acids in patients with low lesions and intermediate when compared to patients without lesions (p <0.05). ECHDC3 expression was 1.2 fold-higher in patients with low lesions than in patients without lesions (p = 0.023), and 1.8 fold- lower in patients with major lesions than in patients without lesions (p = 0.020). In the second casuistry, the expression of ECHDC3 was 1.93 fold-higher in patients with intermediate lesions than in low lesions (p = 0.011) and 1.91 fold-higher in patients with severe lesions than in low lesions (p = 0.013). Spearman's analysis showed a positive correlation between Friesinger's index and ECHDC3 expression (r = 0.327, p = 0.037), but negative with zinc intake (r = -0.317, p = 0.044). The expression of ECHDC3 was positively correlated with the percentage of visceral fat (r = 0.416, p = 0.009) and the percentage was positively correlated with the protein (r = 0.392, p = 0.015). Protein intake had a strong correlation with zinc intake (r = 0.807, p <0.001). Conclusions: It was verified that oleic acid serum level, ECHDC3 expression, percentage of visceral fat, protein consumption may be involved with the progression of CVD, while lower zinc intake could influence the extent of coronary lesion.

MEMBROS DA BANCA:
Interno - 2306763 - KARINE CAVALCANTI MAURICIO DE SENA EVANGELISTA
Externo à Instituição - MARIA ADERUZA HORST - UFG
Presidente - 1804884 - VIVIAN NOGUEIRA SILBIGER