Banca de DEFESA: RODRIGO ALBERT BARACHO RÜEGG

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : RODRIGO ALBERT BARACHO RÜEGG
DATA : 05/09/2016
HORA: 14:00
LOCAL: Sala de aula 02 do Núcleo de Estudos em Saúde Coletiva - NESC/UFRN
TÍTULO:

Analysis of retinol concentration in chickens livers submitted to different thermal processing.

PALAVRAS-CHAVES:

HPLC, retinol, liver, chicken, cooking, freezing.

PÁGINAS: 100
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Brazil has been developing the production and consumption of chicken giblets, especially the liver, which are excellent sources of micronutrients, considered a rich source of vitamin A. This vitamin is a micronutrient that plays an essential role in vision, growth, in the development and maintenance of epithelial tissue, in immunological processes and reproduction. Being a food of easy acquisition and low commercial value, the chicken liver is a bet to combat vitamin A deficiency (VAD), which affects mainly children and pregnant women in developing countries. This study aimed to analyze the concentration of vitamin A by high performance liquid chromatography (HPLC) in livers of farms of chickens, organic and free-range in different conditions of thawing and cooking, marketed in the city of Natal / RN . In addition, this study aimed to validate an adaptation of the methodology proposed by Hosotani & Kitawaga (2003) for retinol analysis in this food so that the method of analysis become simple, fast and cheap. The mean values of retinol in farm chicken liver for the three brands analyzed were: 9152.9 ± 719, ± 389 4673.1, 5943.6 ± 614 mg / 100 g. The average retinol organic chicken liver was 3401.33 ± 597.12 g / 100 g. The average retinol in hillbilly chicken liver was 30 094,79 ± 4628.75 g / 100 g. It was observed that the baking oven for 35 minutes at 200 ° C and thawing process in microwave for one minute caused significant losses of 39.9% and 26.2%, respectively, the retinol concentration in chicken liver farm. The developed and validated assay technique resulted in a rapid extraction and determination and quantification of retinol in chicken liver samples, with an average retention time of 5.2 minutes at 23 ° C, with excellent results linearity (R = 0.9999 ), standard stock stability and freeze-thaw process, accuracy with coefficient of variation below 15% and recovery with values from 93 to 101.2%. Despite significant losses in thermal processes, consumption of an average serving of roasted liver (88g) supplies the daily requirement of vitamin A for a man over 14 years up to 6 times and 20 times the daily needs of children 1 to 3 years. Thus, the chicken liver proved to be an excellent source of vitamin A, with great public health importance in combating VAD, and an affordable and easy food to purchase.

MEMBROS DA BANCA:
Externo à Instituição - HERYKA MYRNA MAIA RAMALHO - UNP
Externo ao Programa - 2578592 - KARLA DANIELLY DA SILVA RIBEIRO RODRIGUES
Presidente - 180.956.404-25 - ROBERTO DIMENSTEIN - UFRJ