Banca de QUALIFICAÇÃO: RODRIGO ALBERT BARACHO RÜEGG

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : RODRIGO ALBERT BARACHO RÜEGG
DATA : 07/06/2016
HORA: 14:00
LOCAL: Núcleo de Estudos em Saúde Coletiva (NESC/UFRN) - sala da aula 01
TÍTULO:

Analysis of retinol concentration in chicken liver trades in large supermarkets of Natal/RN.

PALAVRAS-CHAVES:

HPLC, retinol, liver, chicken, nutritional deficiency, food composition table.

PÁGINAS: 115
GRANDE ÁREA: Ciências da Saúde
ÁREA: Nutrição
RESUMO:

Brazil has been developing the production and consumption of chickens and viscera are excellent sources of micronutrients, especially the liver, considered a rich source of vitamin A. Vitamin A is a micronutrient that plays an essential role in vision, growth, in the development and maintenance of epithelial tissue, in immunological processes and reproduction. Being a food easy acquisition and low commercial value, the chicken liver is a bet to combat vitamin A deficiency (VAD), which affects mainly children and pregnant women in developing countries. This study aimed to analyze the concentration of vitamin A in livers of chickens farms, organic and rednecks at different storage conditions, thawing and cooking, marketed in the city of Natal / RN. In addition, this study aimed to validate an adaptation of the methodology proposed by Hosotani & Kitawaga (2003) for retinol analysis in this food. The chicken liver was homogenized and subjected to extraction and analysis of retinol by high-performance liquid chromatography (HPLC), as proposed by Rüegg & Dimenstein (unpublished work). The average was considered significant when p <0.05. In Article 1 were observed the mean retinol levels in chicken liver for the three brands analyzed: 9152.9 ± 719th, 4673.1 ± 389b, and 614c ± 5943.6 g / 100 g, differing significantly (p <0.01) and are greater than those found in other food composition tables, as TACO (2011), USDA (2008), IBGE (2008-2009) and Franco (1998). Article 2 was observed that the baking oven for 35 minutes at 200 ° C and thawing process in microwave for one minute caused a significant loss of 39.9% (p <0.05) and 26.2% (p <0.01), respectively, the retinol concentration in hen liver. Differences were found between the studied brands and between different cooking processes and thawing, however, the values still found characterize the liver as an excellent source of retinol. Alert to to respect the tolerance limit of 3000 mg / day of vitamin A intake to avoid possible teratogenic effect. Article 3 is a modification of the method was developed and validated using an isocratic procedure C18 column (5 mm, 250 x 4.6 mm) reverse phase system, coupled to a SPD-20A UV-VIS detector, length wave at 325 nm and with only one mobile phase: methanol. The method developed is shown inexpensive and simple, allowing a rapid extraction, determination and quantification of retinol in chicken liver samples, with an average retention time of 5.2 minutes at 23 °C, yielding good results linearity R = 0,9999, standard stock stability and freeze-thaw process, with precision coefficient of variation lower than 15% and recovery with values from 93 to 101.2%. Organic chicken liver retinol showed results in the range of 3401.33 ± 597.12 mg / 100 g, as the hillbilly averaged 30094.79 ± 4628.75 g / 100 g, the latter showed a significant difference ( p <0.001) when compared with organic farm and livers. Furthermore, no significant loss liver subjected to cooling and freezing for up to 10 and 30 days, respectively. There was a large amount of retinol in farm chicken livers, organic and rustic, showing that food is an excellent source of vitamin A.

MEMBROS DA BANCA:
Interno - 2373959 - LARISSA MONT ALVERNE JUCA SEABRA
Externo ao Programa - 302225 - NELY HOLLAND
Presidente - 180.956.404-25 - ROBERTO DIMENSTEIN - UFRJ