Banca de DEFESA: LEA ISIS MARTINS GOMES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LEA ISIS MARTINS GOMES
DATE: 31/05/2023
TIME: 09:00
LOCAL: Virtual (Google Meet)
TITLE:

Evaluation of the antiviral effect of Zinc in SARS-CoV-2 infection model

KEY WORDS:

Evaluation of the antiviral effect of Zinc in SARS-CoV-2 infection model

PAGES: 30
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUMMARY:

Infection with SARS-CoV-2 can trigger an exacerbated inflammatory response, characterized by excessive activity of the immune system in an attempt to protect the organism from the pathogen. Upon recognition of viral particles, a very aggressive immune response is triggered, which may cause immunopathologies mediated by the release of pro-inflammatory cytokines, in addition to cytopathic events in infected cells. Zinc (Zn) is an essential micronutrient, which plays important physiological roles in cellular processes, from the immune response, signal transduction, organelle homeostasis to cell proliferation, and is already well known for its antioxidant, anti-inflammatory,  immunomodulatory and antiviral. Some studies relate the antiviral role of Zn to immunity, linking its effectiveness against COVID-19. The antiviral properties of Zn can occur from reducing viral infection, inhibition of viral protease and polymerase. Thus, the objective of this study was to evaluate the antiviral action of Zn and to analyze its therapeutic potential in cells of Calu-3 lineages infected by SARS-CoV-2, as well as to verify the role of oxidative stress in culture of pulmonary epithelial cells infected by Zn. SARS-CoV-2. For this, an in vitro assay was performed, in which Calu-3 cells were infected with SARS-CoV-2 and treated with different concentrations of Zinc and Cobalt, which was used as a codependent control, and cells and supernatant were collected for evaluation of viral load, cell viability, inflammatory parameters and oxidative stress. Among them, the cytotoxicity assay, LDH dosage, cytokine dosage, oxidative stress dosage through flow cytometry and viral load quantification by plaque assay (PFU). Our data demonstrate that Zn was able to inhibit viral replication in Calu-3, in addition to having an anti-inflammatory effect by decreasing IL-6 and TNF-a production, decreasing oxidative stress and cell death profile. These data suggest that Zn has potential antiviral effect as an adjuvant therapy and may provide protection by decreasing lung inflammation.

COMMITTEE MEMBERS:
Externa ao Programa - 3313589 - JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS - nullExterno à Instituição - KLEBER JUVENAL SILVA FARIAS
Presidente - 1801992 - PAULA RENATA LIMA MACHADO