Banca de QUALIFICAÇÃO: LARISSA HENRIQUE PONTES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LARISSA HENRIQUE PONTES
DATE: 05/05/2023
TIME: 14:30
LOCAL: Virtual (Google Meet - https://meet.google.com/fit-qbqy-bcr)
TITLE:

EVALUATION OF AN ARTIFICIAL FEEDER FOR Lutzomyia longipalpis AND COMPARISON OF CHARGING PATTERNS FOR CDC TRAP BATTERIES.

KEY WORDS:

Sandflies, Lutzomyia, clean energy, artificial feeding.

PAGES: 45
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUMMARY:

Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) is the main vector of the protozoan Leishmania infantum in Brazil and is monitored by surveillance centers, such as the Centro de Controle de Zoonoses (CCZ) in Natal-RN. In the field, monitoring of this important vector is carried out from collections using the Centers for Disease Control (CDC) trap. Laboratory maintenance, on the other hand, requires the ingestion of blood, and it is common to use some vertebrate, which can pose risks to insectary safety and cause damage to the food source. This study sought to standardize an artificial feeding model for Lu. longipalpis in a laboratory environment and enable the use of solar energy to operate the CDC traps. In the present study, common foodstuffs found in the literature and used in mosquito colonies were tested. Additionally, an artificial feeder, Flebfeeding, was built using equipment such as thermostat, boiler, aquarium pump, plastic hoses, water heating reservoir and a flat-bottomed flask. The results showed that the FlebFeeding system was efficient in maintaining blood temperature during food supply and insect attraction, with a feeding success rate of 57% being observed for females. The engorged females laid an average of 19.41 eggs, and the eggs were viable, with larvae and adults hatching in the rearing pots. To evaluate the charging efficiency through solar energy, a charger was built with a 100 W solar panel connected directly to wires connected to the positive and negative poles of a voltage regulator and the battery. Although the charging took longer than the conventional charger, about 18 hours to complete, with an average current of 0.59 A, it was found that the charging was efficient with durability of use in the field similar to the conventionally charged battery. , which required 10 hours to reach full battery charge, with an average current of 1 A. The results indicate that artificial feeding through Flebfeeding is effective for Lu females. longipalpis and has advantages over conventional feeding, as reducing the use of animals in laboratory experiments is an important ethical principle. As for charging CDC trap batteries with photovoltaic panels, it is a viable option and may save public spending in the future. However, research and cost-benefit analysis are needed to assess the feasibility of these techniques on a larger scale.

COMMITTEE MEMBERS:
Interno - 2611909 - CARLOS RAMON DO NASCIMENTO BRITO
Presidente - 1715271 - RENATA ANTONACI GAMA
Externa à Instituição - ROSELI LA CORTE DOS SANTOS - UFS