Banca de DEFESA: LUCAS ABRANTES BATISTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCAS ABRANTES BATISTA
DATE: 28/02/2023
TIME: 14:00
LOCAL: Virtual https://meet.google.com/kxz-jucr-mdt
TITLE:

Natural infection by trypanosomatids in different species of animals in municipalities in the state of Rio Grande do Norte.

KEY WORDS:

Trypanosoma cruzi; Leishmania chagasi; kDNA, 18S,

domesticated animals

PAGES: 68
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUMMARY:

Trypanosomatids are uniflagellate protozoans of the Kinetoplastea subgroup that group the genera Trypanosoma and Leishmania, in which several pathogenic species have developed unique mechanisms to regulate their life cycles and adapt to a variety of hosts. The present study aimed to identify natural infection by Trypanosoma cruzi and Leishmania spp. in different species of animals in the state of Rio Grande do Norte, Brazil. Whole blood samples were collected from 206 animals from 3 mesoregions of the rural state and another four bone marrow and lymph node aspirates from dogs in the urban area were used in the study. The DNA extracted from the samples with phenol-chloroform was submitted to three PCR assays with different molecular markers: 18S (SSU) rRNA that detects Trypanosoma spp. and Leishmania spp., kDNA for detection of T. cruzi and HSP70 for detection of Leishmania spp. Trypanosomatid DNA amplified by 18S PCR was detected in 78.1% (164/210) of the samples, with the highest distribution of positive samples in different species of animals observed in the West mesoregion and the highest percentages of positivity observed in Equus caballus with 85.7% (12/14), Bos taurus with 83.3% (5/6) and Ovis aries with 82.0% (50/61). Amplification of the T. cruzi kDNA was observed in 47.1% (97/206) of the whole blood samples of the animals studied, with a greater distribution of positive samples being observed in different species of animals in the western mesoregion, in which Equus caballus and Canis familiaris were the animal species with the highest percentage of positive samples with 57.1% (8/14) and 52.4% (54/103), respectively. HSP70 PCR from Leishmania spp. amplified DNA in 100% of culture samples from urban dogs and was not observed in whole blood samples from rural areas. Positive samples in 18S PCRs and kDNA were compared, and positive samples were observed in all orders of animals, with emphasis on Canis familiaris (43/106), Equus caballus (6/14) and Bos taurus (3/6). The 18S amplified DNAs that resulted in excellent quality electropherograms were from four samples, all obtained from dogs. These sequences were compared with reference sequences, which revealed one DNA from T. cruzi and three from L. infantum. These results revealed a high number and diversity of infected domesticated animals, reinforcing the importance of better understanding the maintenance cycles of the parasites close to human dwellings.

COMMITTEE MEMBERS:
Externo à Instituição - AMANDA SILVA DOS SANTOS ALIANÇA - Ceuma
Presidente - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Externo ao Programa - 1046091 - JOAO FIRMINO RODRIGUES NETO - null