Banca de QUALIFICAÇÃO: VICTORIA RAMÍREZ OROZCO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : VICTORIA RAMÍREZ OROZCO
DATE: 03/02/2021
TIME: 09:00
LOCAL: Vídeoconferência
TITLE:

Evaluation of Biomarkers for chronic chagasic cardiopathy 

KEY WORDS:

Chagas disease, chronic chagasic cardiopathy, patients, CTLA-4, troponin-I, myoglobin.

PAGES: 67
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUBÁREA: Protozoologia de Parasitos
SUMMARY:

Chronic chagasic cardiopathy is observed in 17-50% of patients, contributing to morbidity and mortality of Chagas disease. There are no biomarkers of clinical evolution described for Chagas disease, requiring high-cost clinical examinations (electrocardiogram, echocardiogram, X-ray, opaque enema), which require the presence of specialized professionals and many cases are invasive and uncomfortable to patients. CTLA-4 is expressed in regulatory T cells and is soluble in blood plasma, CTLA-4 expression is influenced by infections. Cardiac proteins released after tissue damage can generate the production of autoantibodies that contribute to the process of cardiac damage. This study aimed to evaluate anti-troponin I, anti-myoglobin autoantibodies and CTLA-4 soluble in serum as non-invasive clinical biomarkers for chronic chagasic cardiophaty. Specific IgG production anti-T. cruzi and autoantibodies anti-troponin I, anti-myoglobin, and soluble CTLA-4 concentration were determined by ELISA in the serum of 90 chronic chagasic patients with indeterminate (n = 30), cardiac (n = 30), digestive (n = 14) and cardiodigestive (n = 16) clinical forms, and correlated with left ventricular ejection fraction, cardiothoracic index and atrial diameter. Sera from uninfected individuals (n = 30) were used as controls. Chagasic patients with indeterminate, cardiac, digestive and cardiodigestive clinical forms had higher production of total IgG anti-T cruzi and anti-troponin I and anti-myoglobin autoantibodies, when compared to uninfected individuals. In addition, cardiac patients showed higher production of anti-myoglobin IgG autoantibodies compared to indeterminate patients. Interestingly, indeterminate patients had higher production of soluble CTLA-4, when compared to patients with cardiac, digestive and cardiodigestive forms. Our results indicate that the high production of anti-troponin-I and anti-myoglobin autoantibodies associated with low soluble CTLA-4 may be associated with the development of the cardiac form of Chagas disease.

BANKING MEMBERS:
Presidente - 1752367 - PAULO MARCOS DA MATTA GUEDES
Interna - 1715271 - RENATA ANTONACI GAMA
Interno - 2213126 - VALTER FERREIRA DE ANDRADE NETO