Banca de QUALIFICAÇÃO: ANA BEATRIZ RAMALHO DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA BEATRIZ RAMALHO DA SILVA
DATE: 12/08/2020
TIME: 09:00
LOCAL: Vídeo conferência
TITLE:

EFFECT OF THE INDOLE ALKALOID CAULERPINE, OBTAINED FROM ALGAE OF GENDER Caulerpa, ON THE MURINE MODELS OF PERITONITIS INDUCED BY ZIMOZAN AND EAR EDEMA INDUCED BY XYLOL

KEY WORDS:

caulerpina, peritonite, zimosan, citocinas, edema de orelha

PAGES: 70
BIG AREA: Ciências Biológicas
AREA: Imunologia
SUMMARY:

Inflammation is an adaptation response of the immune system to harmful stimuli such as pathogens, damaged cells and toxic compounds and acts by removing the harmful stimulus and repairing the damaged tissue. However, when exacerbated, this response can have negative consequences for the individual, such as fibrosis and loss of tissue function. Corticosteroids are the drugs currently used in the treatment of inflammatory diseases. However, its relationship with the appearance of adverse effects has increasingly driven the search for compounds derived from natural products with anti-inflammatory pharmacological properties so that they can be used as a therapeutic alternative. Thus, the present study aimed to evaluate the anti-inflammatory potential of caulerpine, an alkaloid derived from algae of the genus Caulerpa, in murine models of zimosan-induced peritonitis and xylene-induced ear edema. In the peritonitis model, pre-treatment with caulerpine was able to reduce cell migration to the peritoneal cavity at doses of 4, 2 and 1 mg/kg, with the dose of 2 mg/kg being the one that showed the most expressive result among the three doses tested in time 24h. In the analysis of cell migration kinetics, using a dose of 2 mg/kg of caulerpin, inhibition of migration was observed after 6, 24 and 48 h of zimosan inoculum. This effect was related to the decrease in the levels of pro-inflammatory cytokines (IL-6 and IL-1β) in the peritoneal lavage, in the three evaluated times. In a model evaluating edema, it was observed that the three tested doses of caulerpine acted by inhibiting the formation of ear edema induced by xylene, which was demonstrated by the high percentage of inhibition of the process (based on the weight of the ear sections) and the preservation of the tissue morphology of the ears in the animals that received the treatments, with the dose of 2 mg/kg being the most efficient in this inhibition. This study corroborates the last study carried out by our group that showed that caulerpine (4 mg/kg) has a protective effect in a murine model of DSS-induced colitis. This study is important since here we were able to reach the approximate interval of the optimum dose of efficiency of the CLP (2 mg/kg), which was attested in both experimental models used here.

BANKING MEMBERS:
Presidente - 1346635 - JANEUSA TRINDADE DE SOUTO
Interno - 1752367 - PAULO MARCOS DA MATTA GUEDES
Interna - 2121234 - VANIA SOUSA ANDRADE