Banca de QUALIFICAÇÃO: NATHAN RAVI MEDEIROS HONORATO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : NATHAN RAVI MEDEIROS HONORATO
DATA : 11/11/2019
HORA: 14:00
LOCAL: Sala Quixabeira
TÍTULO:

Evaluation of ecological aspects of triatomines and distribution of Trypanosoma Cruzi dtus isolated from vectors captured in different mesoregions of Rio Grande do Norte, Brazil

PALAVRAS-CHAVES:

Triatoma brasiliensis. Peridomicile. Trypanosoma cruzi. Discrete Typing Units. Mixed infection

PÁGINAS: 72
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:

This work evaluated the ecological aspects of triatomines and the distribution of distinct typing units (DTUs) of Trypanosoma cruzi isolated from vectors in different mesoregions of the state of Rio Grande do Norte (RN). Catches of triatomines were carried out in rural areas in 20 municipalities from 2015 to 2019. Thus, the most captured species were evaluated, as well as the rates of infestation, colonization and natural infection. And the isolated parasites were molecularly characterized in DTUs with different markers. The infestation was observed in 6.3% of the intradomiles and 14.2% of the peridomiciles surveyed. The presence of nymphs in the peridomicile was observed in 95.7% (45/47) of infested households, indicating colonization in this environment. A total of 1,016 triatomines were captured in anthropic and wild environments, Triatoma brasiliensis being the most common species, followed by Triatoma pseudomaculata (15.8%), Panstrogylus lutzi (0.5%), Rhodnius nasutus (0.1%). and Triatoma sp. (0.1%). T. brasiliensis was the only species found in the three environments, and also capable of infesting the largest variety of infested ecotopes. Natural infection by T. cruzi was detected in 82 specimens of triatomines, being T. brasiliensis the species with the largest number of specimens (94.0%). DTU I was identified by infecting T. brasiliensis in the three mesoregions studied, and T. pseudomaculata in the western mesoregion. DTU II was found in T. brasiliensis only in the Central mesoregion, both in the peridomicile and in the wild environment. Mixed infections with TcI and TcII were also observed in T. brasiliensis the Central mesoregion. DTU III was detected both in P. lutzi captured in the wild environment and in T. brasiliensis found in the peridomicile, in municipalities of the Central and Agreste mesoregions, respectively. These results show that T. brasiliensis remains the species with the greatest epidemiological importance in the semiarid region of NB, due to its wide geographical distribution, high degree of adaptation to different environments and ecotopes, high rate of natural infection by T. cruzi and the diversity of species. DTUs that can harbor, and reinforce the need for ongoing entomological surveillance in the state in order to prevent contact of this species with humans and domestic animals. In addition, our data reinforce the presence of three DTUs in the state and highlighting the presence of TcI, TcII and TcIII, with wide geographic distribution of TcI. The presence of T. brasiliensis peridomiciliary specimens infected with TcI, TcII and TcIII suggests that this species may be responsible for introducing different DTUs into the peridomiciliary transmission cycle in the newborn.

MEMBROS DA BANCA:
Presidente - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Interno - 1752367 - PAULO MARCOS DA MATTA GUEDES
Interno - 067.948.304-77 - TACIANO DE MOURA BARBOSA - UFRN