Banca de DEFESA: PEDRO HENRIQUE FERREIRA CAVALCANTI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : PEDRO HENRIQUE FERREIRA CAVALCANTI
DATA : 28/02/2019
HORA: 09:00
LOCAL: Sala de reuniões do Centro de Biociências.
TÍTULO:

Genetic Characterization and Phylogeny of HIV-1 in Rio Grande do Norte

PALAVRAS-CHAVES:

HIV, Resistance mutation, Rio Grande do Norte, Phylogeny.

PÁGINAS: 123
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
SUBÁREA: Biologia e Fisiologia dos Microorganismos
ESPECIALIDADE: Virologia
RESUMO:

From the earliest reports of AIDS in the 1980s to the present day, HIV has claimed approximately 35 million lives and is considered one of the worst pandemics ever recorded in humans. HIV is an RNA virus belonging to the family Retroviridae, subfamily Orthoretrovirinae of the genus Lentivirus. Although there are two species of HIV, only HIV-1 has a pandemic distribution. Phylogenetically the virus is divided into several groups, subtypes, sub-types, CRF's and URF's. With the advent of highly active antiretroviral therapy (HAART) HIV infection is considered to be chronic, the well treated individual will not develop AIDS, leading to an improvement in the quality of life and the possibility of non-transmissibility. However, increasing antiretroviral resistance, mainly due to poor adherence to treatment, could hamper the global progress made so far in combating HIV. The objective of this study was to perform the phylogenetic characterization of circulating HIV-1 strains in the State of Rio Grande do Norte from samples from January to October 2018. A total of 170 samples from the four health regions of Rio Grande do Norte were selected, meeting the established inclusion criteria, and in 8 samples it was possible to perform all the methodological steps of the research. The phylogeny was carried out by means of sequencing of the protease and reverse transcriptase regions of the Pol gene, indicating predominance of circulating HIV-1-B with one patient presenting the F1 subtype and one presenting subtype C. The analysis of the genetic sequencing of the viruses indicated the presence of several mutations of resistance, both transmitted and acquired, as well as accessory mutations, only mutations of clinical importance were not detected in 4 samples, the most recurrent mutations were modeled and identified in reverse transcriptase in regions of α-helix, β-sheet and region of shoulder strap. In conclusion, this study revealed a predominance of circulating HIV-1 subtype B and the mutation profile revealed that the most impaired antiretrovirals were reverse transcriptase inhibitors. The information is fundamental for adequate redirection of therapeutic rescue of these patients and molecular epidemiological screening of circulating strains.

MEMBROS DA BANCA:
Externo à Instituição - GONZALO JOSE BELLO BENTANCOR - Fiocruz - RJ
Externo à Instituição - JOAO FELIPE BEZERRA - FANEC
Interno - 348473 - JOSE VERISSIMO FERNANDES
Presidente - 1715230 - JOSELIO MARIA GALVAO DE ARAUJO