Banca de DEFESA: ROGÉRIO DAMIÃO DE SOUZA JÚNIOR

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ROGÉRIO DAMIÃO DE SOUZA JÚNIOR
DATA : 26/02/2019
HORA: 08:30
LOCAL: POP Centro de Convivência
TÍTULO:

Evaluation of the FAMACHA © method for the detection of Haemonchus contortus anemia in goats and sheep

PALAVRAS-CHAVES:

FAMACHA; Goats and Ovine

PÁGINAS: 71
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
RESUMO:

In Brazil, mainly in the semi-arid regions, the control of the gastrointestinal parasites is carried out by the use of anthelmintics, although in many cases without considering the recommendations, leading to the development of parasitic resistance. Thus, the FAMACHA^© method was introduced as a new technique to aid in the parasites control by means of the target selective treatment. Therefore, the aim of the present study was to compare the sensitivity and specificity of the FAMACHA^© method in the detection of Haemonchus contortus anemia in goats and sheep. For this, goats and sheep were evaluated from Embrapa Caprinos e Ovinos and from EAJ/UFRN, composed by 264 crossbred sheep and 256 crossbred goats of both sexes, aged between 3 and 5 months, respectively, kept on pasture native to Caatinga and Tanzania grass for a period between 98 and 225 days, totaling 4568 observations for sheep and 825 for goats. Four standard values of packed cell volume (PCV) ≤15%, ≤18%, ≤19%, ≤22%, were used to confirm anemia. Specificity (Sp) and sensitivity (Se) were determined. The correlation between FAMACHA^©, PCV and egg counts per gram of faeces (OPG) was calculated using the Spearman correlation coefficient. To evaluate the concordance between FAMACHA^© and PCV, the Kappa value (Ƙ) was obtained. At all PCV cut-off points, the FAMACHA^© Se in Embrapa Caprinos e Ovinos sheep increased when FAMACHA^© 3 animals were included as anemic (75% to 92%). Sp was higher in goats than in sheep at all PCV cut-off points when FAMACHA^© 3 animals were not included as anemic (99.4% and 99.8%). For the EAJ/UFRN sheep herd, higher values of Se were observed when FAMACHA^© 3 animals were included as anemic (48.5% to 51%). The Sp of these animals ranged from 88.1% to 90.1% when FAMACHA^© 3 animals were not included as anemic. The evaluation of the FAMACHA^© method for the goats of the EAJ/UFRN was not significant, since the parasite load of H. contortus did not reach the recommended minimum of 60%. The highest values of correct treatments were found at all PCV cut-off points for both Embrapa Capinos e Ovinos herds when FAMACHA^© 4 and 5 were considered positive for anemia (75.7% to 91% for sheep and 82.1% to 98.5% for goats). In the sheep herd of EAJ/UFRN the highest values of correct treatment were found when FAMACHA^© 3 animals were not included as anemic (65.13% to 78.2%). The value for Embrapa Caprinos e Ovinos sheep herd ranged from 0.320 < Ƙ < 0.560, higher when FAMACHA^© 3 was not included as positive for anemia, indicating a reasonable to moderate agreement. For goats, the value Ƙ = 0.620 for the PCV ≤19 was observed, with substantial agreement being obtained. EAJ/UFRN herd, values indicated a reasonable concordance for sheep (0.067 < Κ < 0.074) and poor agreement for goats (Ƙ ≤ 0.02, p> 0.05). These results corroborate the applicability of the FAMACHA^© method in cases of haemocoses in both herds. The PCV ≤15 cutoff point proved to be quite adequate for both species because it has high sensitivity indexes, but studies aiming at a better standardization of PCV cut values in goats are still necessary to increase the Se of the method for this species.

MEMBROS DA BANCA:
Externo à Instituição - ANA CARLA DIOGENES SUASSUNA BEZERRA - UFERSA
Interna - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Presidente - 1714262 - LILIAN GIOTTO ZAROS DE MEDEIROS