Banca de QUALIFICAÇÃO: SARA ESTER DE LIMA COSTA
Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.DISCENTE : SARA ESTER DE LIMA COSTA
DATA : 07/02/2019
HORA: 09:00
LOCAL: Sala de Reuniões do CB
TÍTULO:
Antiproliferative and cytotoxic activity of hydroalcolic extract and essential oil from Alecrim-da-chapada (Lippia gracilis SCHAUER)
PALAVRAS-CHAVES:
PÁGINAS: 65
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:
In discovering new drugs, plants stand out as a source capable of producing safe, high-efficiency products. In this respect, the Brazilian Caatinga is rich in plants with medicinal properties such as those of the Verbenaceae family, which includes the species Lippia gracilis (also known as alecrim-da-chapada), usually used to treat throat and stomach affections. oils and extracts. Therefore, the objective of the present study was to evaluate the cytotoxicity and anti-proliferative activity of the gastric adenocarcinoma cell line (AGS) in vitro against the hydroalcoholic extract (EHA) and essential oil (OE) of L. gracilis (LG). For this, the chemical components present in the essential oil were analyzed through gas chromatography associated with mass spectrometry (GC/MS) and evaluation of cell viability through MTT reduction, clonogenic assay and flow cytometry. The major components of the phytochemical analysis of the essential oil were carvacrol (51.44%) followed by thymol (15.61%), monoterpenes that proved to have antioxidant, anti-inflammatory and antimicrobial action. Cell viability was assessed at 24 h, 48 h and 72 h at concentrations of 125, 250, 500, 750 and 1000 μg/ml for reduction of MTT in both AGS and normal murine fibroblast (3T3) cell lines. Similar cell death for AGS was observed in 48h and 72h and quietly in 24h, both in OE and EHA; 3T3 presented cell death only at the concentration of 1000 μg/ml in the period of 24h, whereas in the periods of 48h and 72h the percentage of death was similar between periods from the concentration of 500 μg/ml. The clonogenic assay (1 x 102 cells/well) was given over a period of 10 days at concentrations of 125 to 500 μg/ml, showing results of 100% reduction of tumor cell proliferation. Finally, flow cytometry was performed using the 48h period and 500 μg/ml concentration, revealing 0.12% cell viability using EHA and 1.09% using OE, whereas the negative control (CN) presented 98%. These results reveal a high anti-proliferative potential when compared to CN, induction of apoptosis and possible anticarcinogenic activity of LG EO and EHA compounds, as well as antioxidant and cytotoxic activity. In view of this, plant extracts and essential oils of L. gracilis have an important anticarcinogenic effect and may be considered a promising candidate for alternative therapy to gastric tissue disorders, if it is validated as a phytopharmaceutical.
MEMBROS DA BANCA:
Presidente - 2121234 - VANIA SOUSA ANDRADE
Externo ao Programa - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externa ao Programa - 1569526 - RENATA MENDONÇA ARAUJO