Banca de DEFESA: MARIA DA CONCEIÇÃO ALEXANDRE CASTRO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARIA DA CONCEIÇÃO ALEXANDRE CASTRO
DATA : 22/02/2017
HORA: 09:00
LOCAL: Sala de reuniões do CB
TÍTULO:

Evaluation of the occurrence of species Candida parapsilosis complex in biological samples of patients and professionals of hospitals Natal/RN.

PALAVRAS-CHAVES:

Candida spp, Candida parapsilosis, Rio Grande do Norte, Brazil, clinical samples, ICU professionals samples

PÁGINAS: 85
GRANDE ÁREA: Ciências Biológicas
ÁREA: Microbiologia
RESUMO:

Among Candida non-albicans species, the Candida parapsilosis complex (C. parapsilosis stricto sensu, C. orthopsilosis and C. metapsilosis) is the most frequente in systemic infections, being found in hands of health professionals, biomaterials and infusions as the main sources of nosocomial infection. Since these species have different virulence and antifungal susceptibility profiles, the species-specific diagnosis may contribute for the most suitable therapeutic choice. The aim of this research was to compare the occurrence of Candida parapsilosis species and the other Candida species in clinical samples isolated by LACEN-RN and Centro de Patologia Clínica de Natal/RN and in samples obtained in hands and buccal mucosa of health professionals from the ICU of Giselda Trigueiro and Monsenhor Walfredo Gurgel hospitals, from august 2015 to September 2015. The species identification was previously performed by Vitek equipment and alternatively by Chromagar, ITS1-5.8S-ITS2 sequencing or PCR of the VMA intein (for distinguishing among the species from C. parapsilosis complex). Among the 163 clinical isolates, C. albicans (54%), C. tropicalis (18%), C. parapsilosis complex (14%) and C. glabrata (7%) were the most prevalent, whereas in the 196 isolated collected from health professionals the most prevalent Candida species were C. albicans (4%), C. parapsilosis complex (4%) and C. tropicalis (1%). Our research shows that C. albicans is still the most prevalent species, but the increase in C. tropicalis infections and C. parapsilosis complex is notorious and worrying. The evaluation of the antifungal susceptibility profile of the different species of the C. parapsilosiscomplex studied here did not show marked differences in antifungal activity. Our data are similar to other researches carried out in Brazil, which report the C. parapsilosis complex as the second or third most frequent among Candida non-albicans in hospital infections, being this the first study on the occurrence of C. parapsilosis complex in the state of Rio Grande do Norte/RN using the VMA intein for species identification. 

MEMBROS DA BANCA:
Externo ao Programa - 1149398 - EVELINE PIPOLO MILAN
Presidente - 1997012 - RAQUEL CORDEIRO THEODORO
Externo à Instituição - REGINALDO GONÇALVES DE LIMA NETO - UFPE