Banca de QUALIFICAÇÃO: MEIRE KARLA MIGUEL CRUZ

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : MEIRE KARLA MIGUEL CRUZ
DATA : 02/02/2017
HORA: 09:00
LOCAL: Sala de Reuniões do Centro de Biociências
TÍTULO:

Innate imune receptors in the canine visceral leishmaniasis

PALAVRAS-CHAVES:

innate immune receptors, Canine visceral leishmaniasis, Leishmania infantum, TOLL like receptors (TLRs), NOD like receptors (NLRs).

PÁGINAS: 70
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
SUBÁREA: Protozoologia de Parasitos
RESUMO:

Dogs are the primary reservoirs of parasites of the genus Leishmania. Innate immune receptors perform early detection of the parasite and lead to specific adaptive response in attempt to control infection. However, few studies have investigated a correlation between the expression of innate immunity receptors and the resistance or susceptibility in dogs infected with Leishmania infantum. The aim of this study was to correlate the clinical status of dogs naturally infected with L. infantum with the expression levels of innate imune receptors: Toll like receptors-TLRs (TLR1-9), Nod Like receptors-NLRs (NOD1 e NOD2), Nalp like receptors-NLRPs (NLRP1 e NLRP3), cytokines (IL1β, IL-6, IL-12, IL-10, TNFα, IFN-γ) and iNOS in the liver using real-time PCR. Liver and serum samples were collected from dogs naturally infected by L. infantum at the Zoonoses Control Center in Natal, Rio Grande do Norte, Brazil. The positivity of dogs for the parasite was determined by reactivity in the ELISA, DPP® tests and imprint of spleen. Subsequently, fifty-seven dogs were clinically evaluated and classified as asymptomatic (n = 19), oligosymptomatic (19) or symptomatic (n = 19). The results demonstrated the increased expression of all Toll Like receptors (TLR1-TLR9), Nod like receptors (NOD1 and NOD2) and Nalp Like receptors (NLRP1 and NLRP3) in dogs naturally infected by L. infantum, compared with uninfected animals. However, symptomatic dogs showed higher expression of TLR1, TLR3, TLR5, TLR7, TLR8 NLRP1, NLRP3, NOD1 and IL-1β than asymptomatic animals, revealing significant up regulation of transcription with disease progression. On the other hand, asymptomatic dogs presented greater cytokine mRNA expression (IFN-γ, IL-12) and iNOS when compared to oligosymptomatic and asymptomatic animals. This study produced new knowledge involving innate immunity receptors (TLRs, NLRs, NLRPs) and cytokines in canine visceral leishmaniasis and could be used as a basis for better understanding of resistance or susceptibility mechanisms in dogs infected with L. infantum, as well as prophilactic strategies to control canine visceral leishmaniasis.

MEMBROS DA BANCA:
Interno - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Externo à Instituição - MANUELA SALES LIMA NASCIMENTO - IINN
Presidente - 1752367 - PAULO MARCOS DA MATTA GUEDES