Banca de QUALIFICAÇÃO: NAYANA LUÍZA SOARES DE ARAÚJO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : NAYANA LUÍZA SOARES DE ARAÚJO
DATA : 03/02/2017
HORA: 09:00
LOCAL: Sala de Reuniões do Centro de Biociências
TÍTULO:

Chemokines expression in patients with different clinical forms of Chagas disease  

PALAVRAS-CHAVES:

Chagas’ disease, Trypanosoma cruzi, chemokines, chemokines receptors, clinical forms.

PÁGINAS: 70
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
SUBÁREA: Protozoologia de Parasitos
ESPECIALIDADE: Protozoologia Parasitária Humana
RESUMO:

Chemokines participate of leucocitary influx in the inflammatory process, which is important important for the development of cardiac and digestive forms of Chagas’ disease. In this study, the mRNA expression of chemokines (CCL1, CCL2, CCL3, CCL4, CCL5, CCL17, CCL22, CCL24, CCL27, CCL28, CXCL9, CXCL10) and chemokine receptors (CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR10, CXCR3) was evaluated in patients with chronic Chagas’ disease, who displayed indeterminate (n=18), cardiac (n=17), cardiodigestive (n=10) and digestive (n=9) clinical forms. The relative mRNA expression was measured by real-time PCR from peripheral-blood mononuclear cells. Patients with the cardiac form displayed a greater mRNA expression of CXCL9, CXCL10, CCR5 and CXCR3, when compared to those who have the indeterminate form of the disease. Patients with digestive form showed a higher mRNA relative expression of CCR3 than cardiac and indeterminate form; there was, also, a positive correlation between this receptor expression and the sigmoid dilation. Patients with cardiodigestive form displayed a higher mRNA expression of CCL5 than patients with the indeterminate and cardiac forms of disease. The chemokines CCL1, CCL2, CCL3, CCL4, CCL17, CCL22, CCL24, CCL27, CCL28, and the chemokine receptors CCR2, CCR4, CCR6, CCR7, CCR8 and CCR10 did not show significant differences in mRNA expression among  the different clinical groups. Our results show that cardiac patients displayed a greater mRNA expression of CXCL9, CXCL10, CCR5 and CXCR3, which are chemokines and chemokine receptors that induce the migration of type 1 response, and possibly contribute to increased myocarditis and heart damage. On the other hand, patients that displayed digestive form of Chagas’ disease showed higher expression of CCR3, chemokine receptor involved in the influx of type 2 response. 

MEMBROS DA BANCA:
Interno - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Externo à Instituição - MANUELA SALES LIMA NASCIMENTO - IINN
Presidente - 1752367 - PAULO MARCOS DA MATTA GUEDES