Banca de DEFESA: EMANUELLE PATRICIA ENRIQUE DA SILVA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : EMANUELLE PATRICIA ENRIQUE DA SILVA
DATA : 28/02/2019
HORA: 09:00
LOCAL: SALA DO COLEGIADO/ DIREÇÃO EAJ
TÍTULO:
LACTOPEROXIDASE POTENTIAL IN THE DIAGNOSIS OF SUBCLINIC MASTITIS IN COWS VIA IMAGE PROCESSING
PALAVRAS-CHAVES:
udder health, immune system, RGB images, principal component analysis.
PÁGINAS: 49
GRANDE ÁREA: Ciências Agrárias
ÁREA: Zootecnia
RESUMO:
Due to the economic damages to dairy cattle in the order of 200 dollars for each cow per year, subclinical mastitis has been increasingly the target of research focused on the control and early diagnosis, since in addition to affecting milk production and composition, modifies the enzymatic concentration, causing losses in the production of derivatives and reduction of shelf life. Lactoperoxidase is one of the enzymes that has its activity altered when the animal is affected with mastitis, so, studies show that there is a positive correlation between lactoperoxidase and somatic cell count (CCS). The objective of this study was to measure the lactoperoxidase quantitatively and qualitatively and, through image analysis, to verify the potentiality of the enzyme in the detection of subclinical mastitis in cows. Fifty samples of milk (34 from Jersey, 08 from Gir and 08 from Guzerá cows) were analyzed for CCS and enzymatic activity. The sample evaluation system consisted in correlating the color presented by the sample with the enzymatic concentration using the RGB (Red - Green - Blue) color system. Data analysis was performed using the statistical platform R 3.5.0. Principal component analysis (PCA) (package Chemometrics With R6 and factoextra7) for sample image data was developed using the resolved absorbance values for RGB channels (normalized via self-scaling). The multiple linear regression for the CCS analysis showed a value of R2 = 0,97 and for the enzymatic activity R2 = 0,96, signaling that the RGB channels increased in intensity according to the increase of the concentration of CCS and lactoperoxidase in the samples, and analysis of the lactoperoxidase by imaging may be indicative of subclinical mastitis in cows.
MEMBROS DA BANCA:
Presidente - 2313454 - ADRIANO HENRIQUE DO NASCIMENTO RANGEL
Externo à Instituição - ANDRE THALER NETO
Externo ao Programa - 1913849 - EDGAR PERIN MORAES