Banca de QUALIFICAÇÃO: CAROLINA LIMA DOS SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : CAROLINA LIMA DOS SANTOS
DATE: 02/10/2025
TIME: 08:00
LOCAL: Sessão pública realizada por meio de Videoconferência
TITLE:

NANOPARTICLES CONTAINING ASTAXANTHIN-PIGMENTED OIL FROM SHRIMP (Litopeneaus vannamei) WASTE MEAL: EVALUATION OF CYTOTOXICITY AND BIOACTIVE POTENTIAL IN VITRO

KEY WORDS:

Shrimp; Waste; Natural pigment; Nanotechnology; Characterization.

PAGES: 71
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

The exponential population growth and the increasing demand for food, especially that of marine origin, have stimulated the search for new technologies. In Brazil, the most cultivated shrimp species is Litopenaeus vannamei. Shrimp processing generates a large amount of waste, composed of the shell and cephalothorax, which accounts for 50% to 60% of the crustacean’s total weight. Improper disposal of this waste can cause serious environmental problems. Astaxanthin is a carotenoid found in particular species such as marine animals, algae, plants, bacteria, and yeasts, and it is present in shrimp waste, offering numerous potential applications in the food industry. Encapsulation is a technological alternative to preserve these bioactive compounds. The main objective of this study was to produce, characterize, and evaluate the cytotoxicity and bioactive potential of nanoparticles containing oil pigmented with astaxanthin extracted from shrimp residue flour. For the analyses, the pigmented oil was obtained from shrimp residue flour. The nanoformulations were produced using the O/W emulsification technique, with porcine gelatin and isolated soy protein as encapsulating agents and Tween 20 as a surfactant. Three formulations were developed: pigmented oil containing astaxanthin and porcine gelatin (EAG), pigmented oil containing astaxanthin and isolated soy protein at a 2:2 w/w ratio (EAGS2:2), and at a 3:1 ratio (EAGS3:1). Nanoparticle characterization was performed using Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), Fourier-Transform Infrared Spectroscopy (FTIR), evaluation of the encapsulation efficiency (%) of the pigmented oil and astaxanthin, Thermogravimetric Analysis (TGA), and Differential Scanning Calorimetry (DSC). The inhibitory effects on glucose metabolism enzymes—α-amylase, amyloglucosidase, and α-glucosidase—were assessed, along with the antioxidant capacity of the astaxanthin-pigmented oil and the nanoparticles through ABTS, reducing power, and hydroxyl radical scavenging assays.SEM micrographs showed that the individual particles had a physical size ranging from 100 to 200 nm, with smooth surfaces and spherical shapes, and tended to aggregate into larger clusters. The average sizes obtained by DLS were 159.68 (14.42) nm for EAG, 1004.97 (151.10) nm for EAGS2:2, and 95.41 (17.83) nm for EAGS3:1. FTIR analysis revealed an enhancement of the O–H and C H stretching bands (3310 cm⁻¹, 3300 cm⁻¹, 3310 cm⁻¹) in EAG, EAGS2:2, and EAGS3:1, respectively, indicating intramolecular interactions between the astaxanthin-pigmented oil and the encapsulating materials. The pigmented oil yield was 65.9%±, with an encapsulation efficiency of 98%, and astaxanthin quantification of 13.96 (1.24) μg/g of oil. Regarding glucose metabolism enzyme inhibition, the pigmented oil (41.03%±6.44) and the nanoformulations EAGS2:2 (44.17%±3.93) and EAGS3:1 (34.1%±1.87) showed inhibitory effects on the enzyme amyloglucosidase. In contrast, significant inhibition of α amylase was observed only in the EAGS2:2 (41.1%±14.3) and EAGS3:1 (35.8%±20.48) formulations (p < 0.05). The EAGS2:2 formulation exhibited the highest ABTS antioxidant capacity, whereas the EAG formulation demonstrated the best reducing power. So far, the EAGS2:2 nanoformulation has proven effective in protecting and enhancing the functionality of the pigmented oil, offering promising alternatives for developing new functional and nutraceutical food ingredients and products.

COMMITTEE MEMBERS:
Externa ao Programa - ***.588.434-** - ANNA BEATRIZ SANTANA LUZ - UFRB
Presidente - 2378605 - CRISTIANE FERNANDES DE ASSIS
Externo à Instituição - SÉRGIO DANTAS DE OLIVEIRA JÚNIOR - EMBRAPA
Interna - 2275877 - THAIS SOUZA PASSOS