Banca de DEFESA: EMANUELA DE LIMA VIANA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : EMANUELA DE LIMA VIANA
DATE: 28/03/2025
TIME: 09:00
LOCAL: Sessão pública realizada por videoconferência
TITLE:

CHARACTERIZATION AND EVALUATION OF PHYSICOCHEMICAL AND BIOACTIVE PROPERTIES OF SEED AND LEAF OILS OF Moringa oleifera

KEY WORDS:

Antioxidant capacity. Antibacterial activity. Cytotoxic activity. Fatty acids.

PAGES: 82
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Moringa oleifera is a plant widely recognized for its bioactive properties. It stands out as a promising source of vegetable oils extracted from its seeds and leaves since using oils for various applications has become increasingly frequent and of relevant industrial application. However, its physicochemical characteristics, bioactive properties, and cytotoxicity have not been studied much. Given the above, the objective of this study was to evaluate the physicochemical characteristics and bioactive properties of the seed and leaf oils of Moringa oleifera. Initially, the oils were analyzed by Hydrogen Nuclear Magnetic Resonance (1H NMR) and Fourier Transform Infrared Spectroscopy (FTIR), followed by determination of the fatty acid profile by gas chromatography coupled to mass spectrometry (GC-MS) and physicochemical characterization of the samples. The oils were analyzed using thermogravimetry (TG) and differential thermal analysis (DTA). The cytotoxicity of the oils was evaluated in vitro using Chinese hamster ovary (CHO) cells and human hepatocarcinoma (HepG2) cells using the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Different fractions of the seed and leaf oils were used to determine the total phenolic compounds (TPC) and bioactive activities. Total Phenolic Compounds (TPC) concentration was evaluated using the Folin-Ciocalteau method. The antioxidant activity was determined by the scavenging of DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azinobis 3-ethylbenzothiazoline6-sulfonic acid) radicals, and Total Antioxidant Activity (TAC). The inhibition of glucose metabolism enzymes in vitro was determined for αamylase, α-glucosidase, and amyloglucosidase. The results of the characterization and physicochemical parameters revealed that both oils have a composition rich in unsaturated fatty acids, with 70.9% for the seed oil and 70.6% for the leaf oil, with emphasis on linoleic acid, which represented 51.7% in the seed oil and 51.5% in the leaf oil. The cytotoxicity analysis revealed that the seed and leaf oils did not have a cytotoxic effect on CHO-K1 and HePG2 cells, with cell viability more significant than 70%. The methanolic fraction of the seed oil presented a TFC of 90.92 µg EAG.g-1 and in vitro antioxidant capacity of 16.82, 9.13 µmol TE.g-1, and 81.33 mg AA.g-1, according to the DPPH, ABTS and CAT assays, respectively. Regarding the leaf oil, its methanolic fraction presented a TPC of 173.18 µg EAG.g-1 and in vitro antioxidant capacity of 19.99, 11.20 µmol TE.g-1, and 56.43 mg AA.g-1 for the same tests. The integral fraction of both oils showed lower TPC values and antioxidant activity than the methanolic fractions. According to the methodology, there was no inhibition for the bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, and Salmonella typhimurium. Still, both oils showed antifungal activity against Candida albicans with minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of 10 mg/mL. Finally, the methanolic fractions, integral fractions, and the insoluble precipitate of the oils showed in vitro enzyme inhibition activity, emphasizing the integral fraction. This fraction demonstrated the highest inhibition percentages, reaching 97.94% for α-glucosidase and 95.32% for amyloglucosidase in the seed oil. For the leaf oil, the values observed were 94.79 and 85.96%, respectively. Therefore, the results show that both oils have potential applications in the food, cosmetics, and pharmaceutical industries.

COMMITTEE MEMBERS:
Presidente - 2378605 - CRISTIANE FERNANDES DE ASSIS
Externa ao Programa - 1517220 - MARCIA REGINA DA SILVA PEDRINI - nullExterna à Instituição - MARIA GIOVANA BINDER PAGNONCELLI - UTFPR