Banca de DEFESA: ANA KAROLINY XAVIER DE GOIS SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA KAROLINY XAVIER DE GOIS SILVA
DATE: 25/03/2025
TIME: 14:00
LOCAL: Sala de Reuniões do Departamento de Nutrição – UFRN
TITLE:

Effect of hyperalimentation and trypsin inhibitor isolated from tamarind seeds on the occurrence of behavior disorder in obese zebrafish

KEY WORDS:

Obesity. Protease inhibitor. Anxiety. Social behavior. Etress

PAGES: 64
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Excessive food consumption is recognized as a risk factor for obesity, a chronic and multifactorial condition. With diagnosis, in addition to lifestyle changes, obesity treatment includes the use of medications, which, in combination with the adoption of new eating habits, can help sustain weight loss. The trypsin inhibitor isolated from tamarind seeds (TTI) is known for its satietogenic and anti-inflammatory properties, and has been shown to be a candidate for evaluation in the treatment of obesity. The present study aimed to evaluate the effect of hyperalimentation and TTI in triggering behavioral disorders in obese zebrafish. Thus, the isolation of TTI and its characterization regarding molecular mass and antitryptic activity were initially reproduced. Next, the effect of feeding Artemia sp., offered in different quantities in the presence and absence of TTI, on triggering anxiety-like behavior (new tank test), social behavior (school preference test) and acute stress (conspecific alarm substance exposure test) in obese zebrafish was evaluated. Zebrafish (N=60) with obesity, hyperfed (OH) (Artemia sp.: 120 mg wet weight/fish/day) and normal-fed eutrophic (EN) (Artemia sp.: 75 mg wet weight/fish/day) were compared regarding behavioral disorder aspects. Furthermore, during the 10 days of the experiment, obese fish were treated with TTI (25 mg/L) (OH+TTI, n=15), remaining hyperfed, and with TTI (25 mg/L), while being normofed (ON+TTI, n=15). On the 11th day of the experiment, assessments were carried out and all behaviors were recorded using a Logitech HD Webcam, and the videos were analyzed using the ANY-maze software. Significant differences were observed in the anxiety type test, in the average moving speed parameter between the fish in the OH and OH+TTI groups (p=0.01), as well as between the ON and OH+TTI animals (p=0.01), in addition to differences in the time at the top parameter, between the fish in the EN and OH+TTI groups (p= 0.03). In the acute stress test generated by alarm, a significant difference was observed in the immobile time parameter between the fish in the EN and OH+TTI groups (p=0.01). In the sociability test, the difference was between animals in the EN and ON+TTI groups (p=0.01), in the average speed parameter. It is concluded that not enough changes were observed to indicate the triggering of anxiety, sociability and acute stress behavior, influenced by overfeeding with Artemia sp. or by TTI in zebrafish with obesity, since significant differences were isolated, occurring in one or another parameter, within each test performed. Therefore, under the conditions tested, TTI presented itself as a candidate for the treatment of obesity that did not promote the aforementioned behavioral disorders.

COMMITTEE MEMBERS:
Externa ao Programa - 1644341 - ANA CAROLINA LUCHIARI - nullPresidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externa à Instituição - ANA LUISA PIRES MOREIRA - UFPB
Externa à Instituição - RICHELE JANAINA ARAUJO MACHADO - UNICHRISTUS