Banca de QUALIFICAÇÃO: ALINE LOPES MARQUES DE SOUSA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ALINE LOPES MARQUES DE SOUSA
DATE: 23/12/2024
TIME: 14:00
LOCAL: Departamento de Nutrição da UFRN
TITLE:

Evaluation of the effect of trypsin inhibitor isolated from tamarind seeds on lipase activity: an in vitro and in vivo study

KEY WORDS:

Peptides. Protease Inhibitors. Tamarindus indica. Obesity. Zebrafish

PAGES: 78
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Obesity is a multifactorial disease with numerous targets for therapeutic intervention. In this context, various peptides and proteins have been the focus of research due to their ability to influence body weight regulation. Thus, this study aimed to investigate the effect of the trypsin inhibitor isolated from tamarind seeds (ITT) on lipase activity both in vitro and in vivo using a zebrafish (Danio rerio) animal model. To achieve this, ITT was first obtained through affinity chromatography using trypsin-Sepharose 4B. It was then characterized for its antitryptic activity, protein quantification, and molecular mass. Subsequently, an in vitro inhibition assay was performed against porcine pancreatic lipase, and the half-maximal inhibitory concentration (IC50) and inhibition constant (Ki) of ITT against lipase were determined. The preclinical study involved diet-induced obese zebrafish. The fish were divided into five groups: eutrophic and normofed animals without treatment; obese and hyperfed animals without treatment; obese and hyperfed animals treated with Orlistat (50 mg/kg Orlistat); obese and hyperfed animals treated with ITT (25 mg/L ITT); and obese and normofed animals treated with ITT (25 mg/L ITT). After 10 days of experimentation, the groups were evaluated for lipase activity, body weight, and lipid profile. ITT achieved 83% lipase inhibition. Furthermore, the IC50 was estimated at 1.59 x 10⁻⁹ mol.L⁻¹, and the Ki was 2.38 x 10⁻⁸ mol.L⁻¹, indicating that ITT acts as a mixed inhibitor. In the in vivo experiment, results showed that neither Orlistat nor ITT inhibited lipase activity under the tested conditions. However, ITT-treated hyperfed and normofed animals showed a significant reduction in body weight compared to the control groups. Although no significant differences were observed in total cholesterol, VLDL, LDL, or triglyceride levels among the groups treated with ITT or Orlistat, plasma HDL concentrations were significantly higher in the ITT-treated groups compared to all other groups. Thus, it can be concluded that ITT represents a promising strategy for the treatment of obesity and the prevention of dyslipidemias and cardiovascular diseases.

COMMITTEE MEMBERS:
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externa à Instituição - ANA LUISA PIRES MOREIRA - UFPB
Externo ao Programa - 1149356 - ELIZEU ANTUNES DOS SANTOS - null