Banca de QUALIFICAÇÃO: ASLAN COSTA TRAJANO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ASLAN COSTA TRAJANO
DATE: 16/12/2024
TIME: 14:00
LOCAL: Banca híbrida – presencial no Departamento de Nutrição e remota por meio de link no Google Meet.
TITLE:

Evaluation of toxicity and bioactive potential of nanoparticles containing phenolic compounds from Cantaloupe melon (Cucumis melo L.) in zebrafish (Danio rerio)

KEY WORDS:

Cucurbitaceae. Phytochemicals. Nanotechnology. Inflammation. Animal Behavior.

PAGES: 100
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Obesity is characterized by fat accumulation and is strongly associated with inflammation and other complications. Total phenolic compounds (TPC) have antioxidant and anti-inflammatory effects and are sensitive to environmental conditions, compromising their functionality. Nanoencapsulation aims to increase the stability and bioactive potentiation of these compounds. Toxicological investigation of plant extracts and new materials is crucial and should precede the evaluation of functional properties to minimize harmful effects. This study evaluated the toxicity and bioactive potential of the crude extract of phenolic compounds from Cantaloupe melon (free - CEPC and nanoencapsulated - EPWC) in zebrafish with diet-induced obesity (DIO). Encapsulation was performed by nanoprecipitation in anti-solvent (acetone) using concentrated whey protein (1 crude extract: 4 encapsulating agent w/w) and Tween 80. Acute toxicological screening was performed in embryonic and adult zebrafish exposed to concentrations (25-400 mg/L) of CEPC and EPWC. The bioactive potential of CEPC and EPWC was investigated by evaluating food intake, zoometric, biochemical, histopathological, gene expression and behavioral parameters in zebrafish with diet-induced obesity (DIO) and treated with standard diet (DIO + NOD), CEPC (DIO + CEPC) and EPWC (DIO + EPWC) for 14 days, using as controls: group submitted to DIO without treatment, and to the standard diet from the beginning to the end of the experiment (NOD). CEPC and EPWC showed 50% inhibitory activity (IC50) of the ABTS+ radical at 1.64 (0.14) and 1.09 (0.02) mg/mL, respectively, suggesting the potentiation of the antioxidant activity in EPWC (7-fold). For embryos, the estimated mean lethal concentration (LC50) of CEPC and EPWC was 231.2 (35.9) mg/L and 233.8 (25.4) mg/L, respectively. For adult fish, it was impossible to estimate the LC50. However, signs of sub-lethality were evidenced at doses ≥ 100 mg/L, with 50 mg/L selected to evaluate the bioactive potential. The investigation of reactive oxygen species (ROS) in the brains of the animals indicated a significant reduction in oxidative stress in the EPWC group than the control (p < 0.05). In the evaluation of bioactive potential, EPWC (50 mg/L) reduced food consumption by 43% (p < 0.05). The zoometric evaluation indicated significant increases in length in the DIO and DIO + EPWC groups (p < 0.05) and a significant reduction in BMI in NOD and EFPC (p < 0.05). Due to a greater total immobile time, the novel tank test suggested anxious behavior in the DIO control (p < 0.05). The EPWC group spent less time in the tank's bottom area than the DIO and DIO + NOD groups (p < 0.05). In the sociability test, the CEPC and EPWC groups obtained higher means for total distance traveled and average speed in movement than the DIO control (p < 0.05). The results suggest the safe application of CEPC and EPWC at doses < 100 mg/L, with a more pronounced bioactive effect of EPWC in improving oxidative stress, zoometric, and behavioral parameters. However, further studies are needed to better elucidate these investigated compounds' mechanisms and effects.

COMMITTEE MEMBERS:
Interna - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externo à Instituição - DENIS BROOCK ROSEMBERG - UFSM
Interno - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Presidente - 2275877 - THAIS SOUZA PASSOS