Banca de QUALIFICAÇÃO: ANA KAROLINY XAVIER DE GOIS SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA KAROLINY XAVIER DE GOIS SILVA
DATE: 29/11/2024
TIME: 13:30
LOCAL: Sala de Reuniões do Departamento de Nutrição – UFRN
TITLE:

Effect of food and trypsin inhibitor isolated from tamarind seeds on behavioral disorder in diet-induced obesity in zebrafish

KEY WORDS:

Obesity. Protease inhibitor. Anxiety. Social behavior. Etress

PAGES: 55
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Obesity is a chronic and multifactorial condition and is related to changes in behavior, metabolism, genetics, environment and psychosocial aspects. The trypsin inhibitor isolated from tamarind seeds (TTI) is known for its satietogenic and anti-inflammatory properties, and has been shown to be a candidate for evaluation in the treatment of obesity. The present study aimed to evaluate the effect of feeding and TTI on behavioral disorders in obesity in zebrafish. Thus, the isolation of TTI and its characterization regarding molecular mass and antitryptic activity were initially reproduced. Next, the effect of feeding with artemia sp., offered in different quantities in the presence and absence of TTI, on anxiety-like behavior (new tank test), social behavior test (preference for school) and Acute stress (conspecific alarm substance exposure) in obese zebrafish. Zebrafish (N=60) with obesity, hyperfed (OH) (artemia sp.: 120 mg wet weight/fish/day) and normal-fed eutrophic (EN) (artemia sp.: 75 mg wet weight/fish/day ) were compared regarding aspects of behavioral disorder. . And also during the 10 days, the obese fish were treated with TTI (25 mg/L) (OH+TTI,n=15), remaining hyperfed, and with TTI (25 mg/L), being normally fed (ON +TTI,n=15). On the 11th day of the experiment, assessments were carried out and all behaviors were recorded using a Logitech HD Webcam, and the videos were analyzed using the ANY-maze software. Significant differences were observed in the anxiety type test, in the average moving speed parameter between the fish in the OH and OH+TTI groups (p= 0.01), as well as between the ON and OH+I=TTI animals (p= 0.01), in addition to differences in the time parameter at the top, between fish from groups EN and OH+TTI (p= 0.03). In the acute stress test generated by alarm, a significant difference was observed in the immobile time parameter between fish in groups EN and OH+TTI (p= 0.01). In the sociability test, the difference was between animals in the EN and ON+TTI groups (p=0.01) in the average speed parameter. Therefore, it is concluded that no marked and significant differences were observed in anxiety behavior, sociability and acute stress influenced by overfeeding with artemia sp or by TTI in zebrafish with obesity. Therefore, under the conditions tested, TTI presents itself as a candidate for the treatment of obesity that did not promote or intensify the aforementioned behavioral disorders.

COMMITTEE MEMBERS:
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externa ao Programa - 1644341 - ANA CAROLINA LUCHIARI - UFRNExterna à Instituição - ANA LUISA PIRES MOREIRA - UFPB
Externa à Instituição - RICHELE JANAINA ARAUJO MACHADO - UNICHRISTUS