Banca de DEFESA: DANIELLE CAROLINE DA SILVA DIAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DANIELLE CAROLINE DA SILVA DIAS
DATE: 19/06/2024
TIME: 14:00
LOCAL: Link de acesso: https://meet.google.com/syq-atfj-dur
TITLE:

ASSOCIATION OF ZINC WITH CIRCULATING microRNA IN WOMEN WITH INSULIN RESISTANCE

KEY WORDS:

microRNA, insulin resistance, zinc.

PAGES: 62
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

MicroRNAs (miRs) play an important role in regulating multiple processes, including insulin signaling pathways, glucose metabolism, and the inflammatory response. Excess or deficiency of nutrients can modulate the expression of miRs and influence the risk of developing diseases. Impaired insulin secretion and increased insulin resistance play a significant role in the pathogenesis of all types of diabetes mellitus. It is crucial to assess the function of pancreatic β cells and their resistance/sensitivity since insulin resistance (IR) is considered the primary cause of type 2 diabetes development. Zinc is recognized as having an essential role in insulin metabolism, and alterations in this hormone's signaling pathway may be associated with zinc deficiency. However, the relationship between glucose metabolism-related miRs and the body's zinc status is not clear. Therefore, this study aimed to evaluate the association of plasma zinc with the expression of miR-191-5p, miR-188-5p, miR-145-5p, and miR143-3p in women with insulin resistance. This comparative cross-sectional study was conducted with 50 overweight and obese adult/elderly women aged between 18 and 60, attended by the Endocrinology and Metabology Service of the Hospital das Clínicas, Faculty of Medicine, University of São Paulo (USP). The classification of IR was performed according to Stern's criteria. An evaluation of circulating miRs expression, analysis of plasma zinc concentration, biochemical profile, inflammatory parameters, and body composition assessment was conducted. Six percent of the participants had zinc levels below the reference value for plasma zinc deficiency (< 70µg/dL). After dividing plasma zinc values into below or above 90µg/dL, a significant difference in fibrinogen concentration was observed (p=0,016). Additionally, a positive correlation was observed between plasma zinc and serum HDL-c concentration (rho=0,279; p=0,050), and a negative correlation with miR-191-5p (rho=-0,428; p=0,002), miR-145-5p (rho=-0,384; p=0,006), and miR-143-3p (rho=-0,357; p=0,011). MiR-191-5p showed a positive correlation with fibrinogen (rho=0,298; p=0,036) and a negative correlation with insulin (rho=-0,374; p=0,007) and HOMA-IR (rho=-0,296; p=0,037). MiR-188-5p showed a positive correlation with HDL-c (rho=0,307; p=0,040) and a negative correlation with insulin (rho=-0,326; p=0,029). MiR-145-5p showed a negative correlation with waist circumference (rho=-0,282; p=0,049), VAI (rho=-0,286; p=0,047), insulin (rho=-0,342; p=0,015), IL-1β (rho=-0,380; p=0,006), IL-6 (rho=- 0,400; p=0,004), and IFN-γ (rho=-0,328; p=0,020). MiR-143-3p showed a negative correlation with insulin (rho=-0,452; p=0,001), HOMA-IR (rho=-0,327; p=0,021), and IL-6 (rho=-0,334; p=0,018). In the adjusted logistic regression model, high expression of miR-191-5p (OR = 0.06; p = 0.012) was inversely associated with zinc concentration. In the functional enrichment analysis conducted with the list of target genes, gene ontology (GO) terms were significantly enriched. Thus, the present study identified a possible epigenomic interaction of zinc in the biological processes involved in IR, highlighting its importance for medicinal, dietary interventions, or monitoring the adverse effects associated with plasma zinc concentration.

COMMITTEE MEMBERS:
Externa à Instituição - TELMA ANGELINA FARALDO CORRÊA - USP
Presidente - 3218336 - BRUNA ZAVARIZE REIS
Externa à Instituição - Graziela Biude Silva Duarte - USP
Interna - 2306763 - KARINE CAVALCANTI MAURICIO DE SENA EVANGELISTA