Banca de QUALIFICAÇÃO: KARINE LILIAN DE SOUZA
Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.STUDENT : KARINE LILIAN DE SOUZA
DATE: 20/05/2021
TIME: 09:00
LOCAL: Sala virtual
TITLE:
- Título do trabalho: Monitoramemnto da infecção por citomegalovírus em pacientes que realizaram o transplante de células-tronco hematopoiéticas.
KEY WORDS:
cytomegalovirus, hematopoietic stem cell transplantation, PCR
PAGES: 40
BIG AREA: Ciências Biológicas
AREA: Microbiologia
SUMMARY:
Primary infection or reactivation by cytomegalovirus (CMV) is associated with increased rates of morbidity and mortality in patients who undergo hematopoietic stem cell transplantation (HSCT). CMV infection increases risk for other opportunistic infections, graft loss and development of graft versus host disease, due to indirect effects on the immune system. Thus, the aim of the study was to estimate the rate of CMV infection up to 90 days after transplantation in patients who underwent HSCT and to relate it to clinical characteristics. Serum samples were collected fortnightly up to 90 days after transplantation from patients seen at Hospital Rio Grande, Natal, Rio Grande do Norte. The extraction of viral DNA was performed in columns with silica gel membrane, according to the manufacturer's instructions, and CMV detection was performed by nested-PCR, using specific primes. 214 peripheral blood samples were collected from 51 patients. Of these, 60.8% were male and 39.2% female, with a median of 35 years of age. Of the hematological diseases, 29.4% had Acute Myeloid Leukemia (AML), 21.5% Acute Lymphoid Leukemia (ALL), 13.7% Chronic Myeloid Leukemia (CML), 13.7% Lymphomas and 21.5% other diseases hematological. Related allogeneic transplantation was performed in 56.9%, unrelated in 41.2% and autologous in 1.9% of cases. In monitoring CMV infection, it was observed that 15 days post-transplant (D15), 28.6% of the patients were positive, 19.5% in the 30 days post-transplant (D30), 29.3% in the 45 days post-transplant -transplantation (D45), 30.8% in the 60 days after transplantation (D60), 33.3% 75 days after transplantation (D75), and 21.9% in the 90 days after transplantation (D90). This study reinforces the importance of monitoring the early reactivation of CMV in groups of patients undergoing HSCT and that patients who underwent unrelated allogeneic transplantation had earlier viral reactivation compared to related allogeneic transplantation.
Keywords: cytomegalovirus, hematopoietic stem cell transplantation, PCR.
BANKING MEMBERS:
Externa ao Programa - 3313589 - JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS
Presidente - 1801992 - PAULA RENATA LIMA MACHADO
Externa ao Programa - 350753 - VALERIA SORAYA DE FARIAS SALES