SCIENTOMETRICS AND GENETIC DIVERSITY OF Microdesmia rigida (Benth.) Sothers & Prance (Oiticica)
Caatinga, molecular marker, polymerase chain reaction, phytotherapy, literature review.
Microdesmia rigida (Benth.) Sothers & Prance (oiticica) is a native species of the Caatinga biome with significant ecological, economic, and pharmacological importance, highlighting the need to expand knowledge about its scientific production and genetic variability in natural populations. In this context, the present study aimed to analyze the scientific production panorama of the species through a scientometric approach and to evaluate the genetic diversity of a natural population using ISSR markers. Additionally, information related to the extractivism of the species was analyzed using data from the SIDRA/IBGE platform, allowing the contextualization of its socioeconomic importance. The analysis of the scientific literature indicated an increase in the number of publications over the years, with a predominance of studies focused on the medicinal and pharmacological properties and the potential for sustainable use of the species. However, important gaps were also identified, especially regarding the scarcity of studies with a molecular approach and the limited scientific production focused on conservation and the characterization of natural populations. The variability assessment was carried out using 20 individuals, with DNA extraction performed by the Doyle and Doyle (1987) method, followed by PCR amplification. Ten ISSR primers were selected, generating 124 loci, with an average of 12.4 loci per primer. Leaf samples showed adequate quality and integrity for molecular analyses, demonstrating their efficiency as starting material for genetic studies in the species. The polymorphic information content (PIC) values ranged from 0.21 to 0.40, with an average of 0.30, classifying the markers as moderately informative. Diversity indices (h = 0.2784; I = 0.4377) indicated moderate variability, with 100% polymorphic loci, while cluster analysis revealed the formation of groups with different levels of similarity among individuals, suggesting the existence of population structure. Overall, the results demonstrate that, despite the growing scientific interest and the species’ potential for use, there are still significant gaps in knowledge regarding its variability and conservation. Furthermore, the extractivism analysis reinforces the importance of the species for local communities, highlighting the need for strategies that reconcile sustainable use and conservation. Thus, this study provides unprecedented information on the structure of natural populations of Microdesmia rigida, contributing to the advancement of scientific knowledge and supporting future research, especially studies focused on population genetics and species conservation in the Caatinga biome.