Banca de QUALIFICAÇÃO: MARIA LUCIA DE AZEVEDO OLIVEIRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA LUCIA DE AZEVEDO OLIVEIRA
DATE: 30/10/2025
TIME: 15:00
LOCAL: VIDEOCONFERÊNCIA
TITLE:

TOXICOLOGICAL EVALUATION AND THERAPEUTIC EFFECT OF THE HYDROALCOHOLIC EXTRACT OF Momordica charantia ON THE ACUTE INFLAMMATORY RESPONSE INDUCED BY LIPOPOLYSACCHARIDE (LPS)

KEY WORDS:

Momordica charantia; cytokines; nitric oxide; acute toxicity; peritonitis model; RAW 264.7 macrophages; anti-inflammatory activity.

PAGES: 76
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Inflammation is an essential physiological process; however, when exacerbated, it can lead to various pathologies. In this context, natural products with anti-inflammatory potential have gained increasing scientific attention, among which Momordica charantia L. (Cucurbitaceae) stands out as a species traditionally used in folk medicine. Despite its widespread use, studies focusing on the leaves are scarce, particularly regarding their chemical composition, toxicological safety, and anti-inflammatory mechanisms. In this study, the hydroethanolic leaf extract (MCHLE) was chemically characterized by LC–MS/MS to identify bioactive compounds and evaluated for acute and repeated-dose oral toxicity in Wistar rats. Antiinflammatory activity was assessed through in vivo assays using an LPS-induced peritonitis mouse model and in vitro assays with LPS-stimulated RAW 264.7 macrophages. Inflammatory parameters, cytokine secretion, nitric oxide production, and gene expression of mediators and signaling pathways were investigated. Chemical characterization revealed the presence of octopamine, ferulate, vitexin-2-O-rhamnoside, and other bioactive phenolics. Toxicological assessments showed no clinical or behavioral signs of toxicity, with normal hepatic and renal biochemical parameters, alongside reductions in glucose and cholesterol levels and improved antioxidant status. In vivo, MCHLE significantly inhibited leukocyte and neutrophil infiltration and reduced TNF-α and NO production, with efficacy comparable to dexamethasone. In vitro, the extract produced dose-dependent inhibition of TNF-α, IL-1β, and NO, accompanied by downregulation of TNF-α, MAPK, and NF-κB expression. In conclusion, MCHLE exhibits a favorable safety profile and relevant anti-inflammatory activity, modulating both inflammatory mediators and upstream signaling pathways. These findings highlight the therapeutic potential of M. charantia leaves as a promising source of novel anti-inflammatory agents.

COMMITTEE MEMBERS:
Externa ao Programa - 1797182 - ERIKA MARAFON RODRIGUES CIACCHI - nullExterna à Instituição - GISELE CUSTODIO DE SOUZA - UEAP
Presidente - 1055045 - MARCELA ABBOTT GALVAO URURAHY