OBTAINING BIOMATERIALS FROM TANNIN/GLYCOSAMINOGLYCANS-BASED DERIVATIVES FOR APPLICATIONS IN TISSUE ENGINEERING AND REGENERATIVE MEDICINE
Polyelectrolyte multilayers, Glycosaminoglycans, Tannins, Scaffolds, Stem cells
Develop stable polysaccharide structures with biochemical and biomechanical properties used by extracellular matrix (ECM) is one of the goals of tissue engineering and regenerative medicine. Improve the biocompatibility of conventional materials through surface modification has been shown to be an effective way to enhance their biological response. We report a new class of layered surfaces to increase biocompatibility and cellular response of biomaterials using tanfloc and glycosaminoglycans (chondroitin sulfate or heparin) since these polymers possess inspired chemistry structures to those found in ECM. The construction of polyelectrolyte multilayers was monitored by Fourier Transform Surface Plasmon Resonance (FT-SPR). The surface properties were assessed by X-ray Excited Photoelectron Spectroscopy (XPS), Atomic Force Spectroscopy (AFM) and contact angle measurements. The response of human adipose-derived stem cells (ADSCs) was evaluated in vitro through cytocompatibility assays, adhesion and proliferation of the cells using fluorescence microscopy. The interactions of ADSCs with these new biomaterial surfaces demonstrate that these surface coatings exhibit good cytocompatibility and that they promote cell attachment and proliferation. These characteristics are very desirable for biocompatible coatings for applications such as tissue engineering scaffolds and orthopedic implants.