Banca de DEFESA: JOSÉ GIOVANI NOBRE GOMES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE: JOSÉ GIOVANI NOBRE GOMES
DATA: 12/06/2015
HORA: 08:00
LOCAL: CONSEC - CCS
TÍTULO:

ESTUDOS DE ASSOCIAÇÃO DOS POLIMORFISMOS GÊNICOS PARA INTERLEUCINA 17 (IL-17)  E FOXP3 EM MUCOSA GENGIVAL E VAGINAL DE MULHERES ADULTAS.

PALAVRAS-CHAVES:

Gingivitis, Polymorphism Genetic, Interleukin-17, Foxp3, Uterine Cervicitis.

PÁGINAS: 110
GRANDE ÁREA: Ciências da Saúde
ÁREA: Odontologia
RESUMO:

Background: By definition, the mucous membranes are responsible for the interface with the outside world, specifically responding to external threats of pathogens. Taken together, the normal flora exerts profound effects on the immune system of the mucous membranes, and likely plays important roles in human physiology and pathology. Recently it was identified that the role of two subpopulations of helper T cell [Treg; forkhead box P3 positive (Foxp3)] and Th17 cells [interleukin-17-positive (IL-17)] maintains strong relationship with periodontal disease (PD), but its relationship with gingivitis needs to be clarified. Tregs are crucial in maintaining peripheral tolerance and regulation of immune response. Th17 cells play a critical role in several autoimmune diseases, inflammation and host defense. The microbial community is one component of a complex set of interactions that plays a vital role in maintaining women’s health. The immune response to vaginal microbiota is poorly understood. To our knowledge, nothing has been published associating the polymorphism of IL -17 and Foxp3 with vaginal microbiota. Objective: The aim of this study was to identify polymorphic variants in the promoter region of the Foxp3 gene and IL-17A and their possible correlation on the severity of gingivitis, thus as, to associate microbial flora and vaginal ecosystem and the role of polymorphic variants in the promoter region of the Foxp3 gene and IL-17A in cervical cytology (Pap smear), besides the association with degree of inflammatory smears, clinical and laboratory data in the northeastern population from Brazil. Methods: Analytical and cross-sectional study with clinical research, conducted through the clinical and laboratory data of conditions gingival and cervicals  of a group of women adultas. Os clinical periodontal parameters evaluated were: plaque index (PI), gingival index (IG) and Periodontal Screening and Recording (PSR). Based on the Gingival Index (GI) values , the subjects were classified according to the severity of gingival inflammation. Pap smears were conducted and it was determined the correlation between the cytological report using the Bethesda system. Inflammatory smears had their intensities classified as mild, moderate and severe. The polymorphisms of FoxP3 and IL-17 genes were analyzed by Restriction Fragment Lenght Polymorphism method (RFLP). DNA samples were obtained from peripheral blood. Data were organized in a database in SPSS ® statistical program and subjected to statistical tests. To assess the degree of association between gingivitis and qualitative aspects was assessed by applying the Chi-Squared test. Fisher and odds ratio (OR) was calculated with the corresponding 95% confidence interval (CI 95%). The analysis of the degree of association of the level of cervical inflammation with qualitative variables we used the Chi-Squared test. The quantitative analysis were applied Kruscal-Wallis and Jonckheere-Terpstra test. Student's t test was used to compare means of GI between the two groups, as well as the One-way ANOVA to compare means between more than two groups. The Hardy-Weinberg equilibrium was assessed by the test chi-square Goodness-of-Fit. Results: The study confirms the high prevalence of gingivitis, with 74 (52.9%) with mild gingivitis and 67 (47.1%) with moderate gingivitis. There was association between the degree of mild gingivitis, with younger than 38 years (OR = 2,618), brushing frequency (p = 0.012), and periodontal screening and recording (PSR) categorization according to grade (p <0.0001) with significant differences in age (p = 0.028), sum of values obtained PSR examined the six sites (p <0.0001) analyzed the number of teeth (p = 0.027) and PI (p <0.001); There were no statistically significant differences between groups with different degrees of gingivitis and the distribution of genotypes and alleles of polymorphisms FoxP3 (CC, CT and TT) and IL-17 (GG, AG and AA). In addition, there were no significant differences between the polymorphism and the clinical parameters of GI. However, an association between the GI and the presence of the C allele in Foxp3 polymorphism (rs3761549) was revealed. The intensity of inflammation of the cervix, 9.8% had a mild inflammation, 35.3% moderate and 46.3% severe. With high prevalence of Gardnerella (20.17%) and Lactobacillus (21.46%). Comparison of distributions of genotypes and alleles of FoxP3 and IL-17 polymorphisms showed no statistically significant differences between groups with different degrees of cervicitis. There is also no significant difference between polymorphisms Foxp3 and IL-17 and microbiological analysis. There was a significant association of the inflammatory intensity with age (p = 0.079), labor income (p = 0.067), use of contraceptives (p = 0.031) and smoking (p = 0.098). Gardnerella vaginalis was associated inflammatory pattern at the expense of other pathogens. Serum estradiol (p = 0.046) showed a significant difference with a lower average when inflammation is greatest. Conclusion: The strongest influences on the degree of gingival inflammation were the amount of biofilm, the frequency of brushing, and age. In addition, the PSR scores were good indicators for evaluating the results. No evidence was found in relation to gum disease with systemic parameters investigated. This first study demonstrated that Foxp3 polymorphisms, particularly single nucleotide polymorphisms (SNPs) involving allele C is associated with the GI. Further studies are needed to characterize these genes more specifically and understand them in more detail, revealing their role in the pathophysiology of gingivitis. Studies  are needed to clarify the molecular aspects of the relationship between Th17 and Foxp3 polymorphism, with potential applications for diseases prediction. The protection role of this molecule may not be excluded. Is required characterize these cells more precisely and to understand, in more details, their roles in the pathophysiology of vaginal ecosystem.

MEMBROS DA BANCA:
Interno - 1199080 - ANA KATHERINE DA SILVEIRA GONCALVES DE OLIVEIRA
Externo ao Programa - 2087759 - ANDRE DUCATI LUCHESSI
Externo à Instituição - EUDES EULER DE SOUZA LUCENA - UERN
Externo ao Programa - 3313589 - JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS
Externo à Instituição - THALES ALLYRIO ARAUJO DE MEDEIROS FERNANDES - UERN