Banca de DEFESA: SARAH DANTAS VIANA MEDEIROS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE: SARAH DANTAS VIANA MEDEIROS
DATA: 17/02/2014
HORA: 14:00
LOCAL: SALA 06 - DEP. DE NUTRIÇÃO E SAÚDE COLETIVA
TÍTULO:

Immunohistochemical evaluation of venous ulcer treated with glucan

PALAVRAS-CHAVES:

Glucan. Venous Ulcer. Tissue Repair. Macrophages. Lymphocytes

PÁGINAS: 73
GRANDE ÁREA: Ciências da Saúde
ÁREA: Medicina
RESUMO:

Glucan is a polymer of glucose that has been reported to accelerate wound healing through the activation of immune cells. Venous ulcers are cutaneous wounds that result from chronic venous insufficiency; they are difficult to heal and responsible for 80-85% of ulcers that occur on the lower limbs. The purpose of this study was to assess the role of the β-glucan in the induction of venous ulcers healing, especially through the identification and quantification of macrophages (CD68⁺ cells) and T-lymphocytes (CD3⁺ CD4⁺ cells and CD3⁺ CD8⁺ cells). A nonrandomized clinical trial with intragroup comparisons over time was performed. The study was approved by the Research Ethics Committee of the University Hospital Onofre Lopes (Nº147/09), Federal University of Rio Grande do Norte. Water-insoluble β-glucan was isolated from Saccharomyces cerevisiae and was dispersed in cream with crodabase CR2 to a final concentration of 3%; the cream was applied directly to the ulcer. A venous ulcer biopsy was taken before therapy initiation (day 0) and after 30 days of glucan treatment for detection and quantification of macrophages (CD68⁺ cells), and T-lymphocytes (CD3⁺ CD4⁺ cells, and CD3⁺ CD8⁺ cells) by immunohistochemistry. We followed 12 patients; nine were women and three were men. The median of the age was 59 years. Topical immunotherapy was applied to 13 venous ulcers. The duration of these ulcers was from 8 to 264 months, and the majority (61.5%) was recurrent wounds. Histomorphometric analysis showed a decrease of the macrophage number in all the cases after the treatment with glucan (p=0.001), and an increase of T-lymphocytes number (p=0.600). Among the cases showed an increase of CD3⁺, 7 (87.5%) also increased CD4⁺ marker, and CD8⁺ marker. Initially, the venous ulcers area varied from 8.38 to 66.89 cm², and after the treatment with glucan the ulcers area varied from 4.84 to 58.02 cm². We concluded that the glucan was able to modulate the immune response, accelerating the healing process of venous ulcers treated. 

MEMBROS DA BANCA:
Presidente - 350753 - VALERIA SORAYA DE FARIAS SALES
Externo ao Programa - 1714290 - RENATO MOTTA NETO
Externo ao Programa - 2330137 - VILANI MEDEIROS DE ARAUJO NUNES
Externo à Instituição - FERNANDA GUEDES LUIZ - NENHUMA
Externo à Instituição - MARIA GORETTI FREIRE DE CARVALHO - UnP