Banca de DEFESA: JOQUEBEDE RODRIGUES PEREIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JOQUEBEDE RODRIGUES PEREIRA
DATE: 31/08/2021
TIME: 09:00
LOCAL: Por Videoconferência
TITLE:

FRAÇÃO DE MANSOA HIRSUTA: ANÁLISE TOXICOLÓGICA, FARMACOLÓGICA, QUÍMICA E DESENVOLVIMENTO DE FILME POLIMÉRICO COMO CURATIVO PARA CICATRIZAÇÃO DE FERIDAS

KEY WORDS:

Keywords: Bignoniaceae; Mansoa hirsuta; Toxicity; Inflammation; Analgesia; Film; Healing;

PAGES: 127
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Mansoa hirsuta D.C. (Bignoniaceae), popularly known as cipó-de-alho, is a native species from
the Brazilian semiarid region and is traditionally used in the treatment of sore throats and
diabetes. Studies report that extracts and fractions of this species have several pharmacological
actions such as antifungal and antioxidant activities, endothelium-dependent vasodilator effect,
and inhibition of the angiotensin-converting enzyme, nitric oxide production, and lymphocyte
proliferation. In this scenario, this study followed research lines: (1) investigating the
composition, toxicity, anti-inflammatory and analgesic actions, and chemical of the fraction
obtained from M. Hirsuta leaves (MHF); and (2) developing chitosan films incorporated with
MHF as an innovative formulation for treating wounds. In vitro cell viability was evaluated in
3T3 cells using the MTT assay. At 24, 48, and 72 h, MHF did not show damage to cell viability.
For the acute toxicity assay, a single dose of the fraction was administered orally in male and
female mice, with no mortality or signs of toxicity. Additionally, in the subchronic toxicity test,
no relevant toxicological changes were observed. The results of the open field and rota-rod tests
showed that MHF did not alter the locomotor activity and behavior of the mice, nor did it alter
the motor coordination and balance of the treated animals. In evaluating the anti-inflammatory
activity, single oral administration of MHF significantly reduced carrageenan-induced edema
and levels of myeloperoxidase. Furthermore, it has decreased the migration of leukocytes and
proteins in the air pouch model. Regarding the antinociceptive activity, it was observed that the
highest doses reduced abdominal contortion in response to the administration of acetic acid and
significantly inhibited the second phase of the formalin test. Ultra-performance liquid
chromatography coupled with a time-of-flight analyzer and mass spectrometry indicated that
this fraction is rich in acid triterpenes that can be derived from oleanolic and ursolic acids. Then,
films were prepared using the solvent evaporation method and characterized by Fouriertransform infrared spectroscopy, X-ray diffraction, thermogravimetry, differential scanning
calorimetry, scanning electron microscopy, and atomic force microscopy. In addition, tensile
strength, elongation at break, and thickness were also measured. Chitosan films containing
MHF (CMHF) exhibited characteristic bands of chitosan and MHF, revealing a physical
mixture of both. CMHF showed amorphous nature, thermostability, and MHF dispersion in the
chitosan matrix, resulting in a rough structure. Incorporation of the fraction into the chitosan
matrix significantly improved the mechanical performance and thickness of the films.
Treatment of wounds in vivo with CMHF for seven days showed a characteristic area of
advanced healing, re-epithelialization, cell proliferation, and collagen formation. Furthermore,
wound closure reached 100% contraction after ten days of treatment with modulation of
interleukins. Therefore, the results of this study indicated that MHF is rich in acid triterpenes
and an anti-inflammatory and analgesic potential without causing acute or subchronic toxic
effects, and could be a promising source to be explored. Furthermore, the incorporation of the
M. hirsuta fraction in chitosan films was advantageous and showed great potential to stimulate
healing and regeneration of wounds.

BANKING MEMBERS:
Externo à Instituição - ADRIANO ANTUNES DE SOUZA ARAÚJO - UFS
Externa ao Programa - 1645202 - ELAINE CRISTINA GAVIOLI
Interna - 1201781 - FERNANDA NERVO RAFFIN
Externo à Instituição - LUCINDO JOSÉ QUINTANS JR - UFS
Presidente - 1306690 - TELMA MARIA ARAUJO MOURA LEMOS