Banca de QUALIFICAÇÃO: NAARA FERRAZ DOS SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : NAARA FERRAZ DOS SANTOS
DATE: 23/04/2025
TIME: 10:00
LOCAL: Videoconferência - Link para acesso: https://meet.google.com/njn-getd-vze
TITLE:

PHYTOCHEMICAL STUDY OF THE AQUEOUS EXTRACT OF Anadenanthera colubrina (Vell.) Brenan SEEDS WITH AEDICIDAL ACTIVITY

KEY WORDS:

Aedes aegypti; Angico; Arboviruses; Botanical bioinsecticides; Mass spectrometry.

PAGES: 69
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Aedes aegypti is a mosquito vector responsible for the transmission of arboviruses such as dengue, zika, chikungunya and yellow fever, which represent a serious public health problem, requiring the development of strategies and measures to control the proliferation of these vectors. In this context, the investigation of botanical extracts from the biodiversity of the caatinga, as a sustainable source of bioactive molecules, represents an alternative for the development of safer and more effective larvicides, promoting health associated with environmental safety. Anadenanthera colubrina is a plant species rich in bioactive compounds, presenting a pharmacological profile with scientific evidence that corroborates its antimicrobial and anti-inflammatory properties. Preliminary studies indicate that aqueous extracts of A. colubrina seeds contain compounds with larvicidal activity against Ae. aegypti. Therefore, aiming to identify the agents involved in the larvicidal activity of this species, in the present study a detailed phytochemical analysis was performed by mass spectrometry. This analysis allowed the annotation of six major biomolecules: pakimic acid, anadanthoside, bufotenin, β-sitosterol, stigmastanol and an anadanthoflavone derivative. The phytosterols β-sitosterol and stigmasterol are known to interfere with the nervous system of insects, acting on acetylcholinesterase. In addition, β-sitosterol and flavonol have been described to interrupt the ecdysis process in insects, which can lead to the death of mosquito larvae. Bufotenin, in turn, is a neuroactive molecule with antiviral and insecticidal potential, although its mode of action is not yet fully understood. Pakimic acid, a kinase inhibitor, can interfere with several cell signaling pathways essential for insect survival. The presence of these biomolecules in the A. colubrina extract suggests that the observed aedicidal activity may be the result of a synergistic action between different compounds, acting on multiple molecular targets. However, further studies, such as in vitro and in silico assays, are needed to elucidate the precise mechanisms of action and the interaction between the different components of the extract.

COMMITTEE MEMBERS:
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo ao Programa - 1893445 - EUZEBIO GUIMARAES BARBOSA - UFRNExterno à Instituição - GIULIAN CÉSAR DA SILVA SÁ